To establish the optimum storage conditions of granulocyte concentrates, the effects of anticoagulants (CPD and ACD) and temperature (22°C and 4°C) on granulocyte functions were examined. Granulocyte concentrates were obtained as buffy coats from fresh blood by centrifugation. The degree of granulocytes damage was studied from cell counts, mean cell volume, morphology, chemotaxis, adhesion, spreading ability, phagocytosis, and bactericidal activity during the storage of granulocytes to 48 hours.
Cell counts and various cell functions were better maintained in CPD than in ACD, and at 22°C than at 4°C during storage. We evaluated synthetically granulocytes compatibility for storage from various cell functions. Granulocytes compatibility (%) was expressed in terms of (% cell counts × % chemotaxis × % phagocytosis × % bactericidal activity)^1/4. All these values were expressed as a percentage to fresh cells. The combination of CPD and 22°C was the most suitable for granulocyte storage. In this condition, stored granulocytes showed on the average 90% and 80% in granulocytes compatibility after 24 hours and 48 hours, respectively. On the other hand, in granulocytes stored at 4°C granulocytes compatibility significantly reduced during 48 hours to below 50% in CPD, and impaired much further in ACD.

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The conditions of granulocyte preservation, and the influences of storage temperature (4°C versus 22°C) and pH had been studied.
Granulocyte counts, mean cell volume, viability and morphology were superior at 4°C-storage. However, chemotaxis, random migration and bactericidal activity were better at 22°C-storage. The decrease in phagocytic activity of granulocytes stored at both temperature was not seen. Adherence ability of granulocytes stored at 4°C or 22°C up to 48hr increased. It seems to be due to damage of plasma membranes. The optimum pH of storage medium was 7.2 for the maintenance of various functions. The pH of granulocyte concentrates collected by ACD-anticoagulant was 7.05, and by CPD was 7.3, respectively.

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