We have obtained one hundred and two miscellaneous various strains of the tubercle bacilli by our own special methods from the circulating blood of the tuberculous individuals and after undergoing in detail studies about these isolated varied strains led us on no account to the following conclusions briefly:—
1) By the injection of the squalin (biological a c tive squalene) into a tuberculous organism we are able to obtain from the circulating blood of the individual directly the so-called colonies of variants of many kinds from the typical tubercle bacilli on the blood agar media, and also able to obtain rarely identical variants from those of the tuberculous patients without the injection of the squalin.
2) The variants cultured on the blood agar media by the injection of the squalin are widely differred in the positive percentages from nearly 100% to 0%, this differences in the positive percentages are considered mainly sprung from the different biochemical natures of the examined squalins. Therefore we have to distingish in the squalene first detected by Mr. M. Tsujimoto into two fundamental biological forms, —the one biochemical active we named it for the sake of convinience as the squalin), the other biochemical almost inactive.
3) The variants of the tubercle bacilli obtained by the injection of the squalin have an obvious tendency to show various forms in the morphological points, namely; granules, cocci, diplococci. tetrads, sarcina, short chains (streptococcoids, diplostreptococcoids), rods in various forms (tiny or stout, short or long, granulous or solid), yeast like large globoid bodies or cocci, filiform bacilli bearing or no branches, sometimes amorphous or homogeneous substances, or dust like, detritus like forms granules and cocci and each form above described also is generally widely varied in its size and figure, to say briefly, the morphological types of the variants of the tubercle bacilli we obtained may review almost entire bacteriological features.
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