Abilities of the polymorphic DNA markers amplified by RAPD (Randomly Amplified Polymorphic DNA) and ISSR (Inter Simple Sequence Repeat) were studied, detected and systematized breeding lines and expanding molecular variabilities and molecular phylogenetic lines of the cultivarietal ‘Marguerites’ of the Argyranthemum lineage and especially the intergeneric hybrids of ‘Marguerite’ X Glebionis carinata Tzvelev or G. coronaria (L.) Spach. The reproducible polymorphic bands were generated by RAPD-PCR using RAPD primer OPC-04, in respective species of Glebionis and the intergeneric hybrids of the ‘Marguerites.’ The primer OPC-04 made it possible to identify either two intergeneric hybrids of ‘Marguerite’ X G. carinata or ‘Marguerite’ X G. coronaria. The polymorphic bands were amplified by ISSR-PCR in the breeding lines and the cultivars of the ‘Marguerite,’ while they were not amplified by RAPD. A specific band was confirmed by ISSR-5 primer in the ‘Marguerite’ cultivar strain of ‘Zairai Shiro.’ This primer ISSR-5 detected and identified certain specific band among the band pattern of ‘Southern Elegance White,’ this cultivar has cv. ‘Early White’ as the paternal parent. Similar polymorphic band pattern was confirmed by ISSR-PCR, in the breeding lines and the cultivars of the ‘Marguerite’ which have similarity in flower color, flowering type and share the hybrid parents.

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  In this study, we have clarified the structural and electronic properties of Cl₆Benzene onto Cu₅(100) cluster using ab initio molecular orbital method. The results obtained in this study are as follows: (1) the result of Mulliken Charge analysis shows that a large number of electrons are transfered from the Cu cluster to the adsorbent, (2) with single-point calculation, transition state exists 4×10^-2 nm away from the adsorption state and this potential energy curve is valid untile 5×10^-2 nm away from the adsorption state, and. (3) Homo-1 and Homo-2 at adsorption state are resulted from orbital interaction between Cu₅ cluster and Cl₆Benzene due to this smallest cluster model.

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As many members come from the wide areas in Aomori prefecture, the exercise from the end of the college year to the early spring, namely for about two months, may be suspended unwillingly. By analysis about how the above exercise suspension influences on the body composition and physical fitness of members of the female basketball club in our college, the following results are obtained : 1) Weight is almost not changed ; 2) Although %Fat is slightly increased, no significance is recognized ; 3) Although the Body Fat Mass is slightly increased, no significance is recognized ; Lean Body Mass is decreased for seven students among ten, and its average is decreased significantly with 5% level ; 4) Vo_2max of all ten members is decreased, and the significant difference is recognized with 0.1% level ; 5) Although the average of the back strength is slightly decreased, no significance is recognized.

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Distinction of intergeneric hybrids between Argyranthemum frutescens (L.) Sch. Bip. and Rhodanthemum gayanum (Coss. & Durieu) B.H. Wilcox, K. Bremer & Humphries is important for the development of new intergeneric hybrids. The aim of this study was to develop a simple and cost-effective DNA marker to distinguish between a large number of putative hybrids of A. frutescens × R. gayanum during primary selection. We designed sequence-characterized amplified region (SCAR) markers based on the genetic sequences of the internal transcribed spacer (ITS) region for A. frutescens and R. gayanum. Specific SCAR markers for A. frutescens amplified bands in A. frutescens and hybrids but not in R. gayanum. In contrast, specific SCAR markers for R. gayanum amplified bands in R. gayanum and hybrids but not in A. frutescens. Additionally, we confirmed the reproducibility of the SCAR marker using 31 cultivars. The findings demonstrated that these SCAR markers could be used to distinguish putative hybrids between A. frutescens and R. gayanum. This is the first report of the development of a SCAR marker to facilitate distinction of these hybrids.

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As many members come from the wide areas in Aomori prefecture, the exercise is suspended unwillingly from the end of the college year to the early spring. By analyses on how the above exercise suspension influences on the body composition, physique and physical fitness of members of the female basketball club in our college, following results are obtained: 1) Weight is almost the same; 2) Although %FAT increases slightly, no significant difference is recognized; 3) FM increases slightly, but no significant difference is recognized; 4) LBM decreases for seven members among ten, and the obvious decrease of the average is recognized at 5% significant level; 5) The average of backstretch decreases slightly, but no significant difference is recognized; 6) V0_2max of all ten members decreases, and the obvious difference is recognized at 0.1% significant level; 7) Ability of all members decreases on how many meters they can run during twelve minutes, and the obvious difference is also recognized at 0.1% significant level; From the above results, followings are confirmed: Viewed from the body composition, nevertheless the rates of increase on %FAT and FM are less than male members, the above exercise suspension causes LBM to significantly decrease similarly to male members; Viewed from physical fitness, achievable distance with running in twelve minutes which is not measured for male members and VO_2max decrease significantly, and obvious influence on heart-lung capability is verified. Suspension causes endurance of physical fitness degradation, careful selection of training menu, therefore, must be considered as re-starting the exercise.

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分子量が1千万を越えるポリアクリルアミドの分子量分布の測定につき検討を加えた. ビニルアルコールコポリマー充填カラム (粒子径9μm, 7.6mm i. d. ×250mml) と移動相として200mMリン酸緩衝液 (pH 6.8) を用いた場合, ピークの保持時間, 重量平均分子量の平均変動係数 (標準偏差を平均値で割った値) はそれぞれ0.13%, 0.69%と良好な結果が得られた. さらに測定時間が15分と短いことから, 分子量が1千万を越えるポリアクリルアミドの品質管理に本法が適用できるものと考える.

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Solid solution perovskite structure of lead zirconate-lead nickel niobate ceramics, (1-x)PbZrO_3-xPb(Ni_(1/3)Nb_(2/3))O_3 (PZ-PNN) with x=0-0.5, were synthesized via the columbite precursor method. The formation of the perovskite phase in the calcined powders has been investigated as a function of calcination conditions by TG-DTA and XRD techniques. The morphology evolution was determined by scanning electron microscopy (SEM) technique.

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For the purpose of radiation protectien against X-ray diffraction apparatus, thc followings were carrried out: (i) Measurements of the exposure rates of the primary and scattered X-rays produced from the apparatus, (ii) Determinations of the sensitivity of many types of surveymeters for the low energy X-rays from the apparatus, and (iii) Experiments on the shielding effect of many kinds of materials such as vinyl sheet, rubber, etc..
Exposure rate of about 600R/min was observed for the primary X-rays at the sample holder of the apparatus under the normal operation. For the 8 keV X-rays emitted from the apparatus, the readings of an ionization chamber type surveymeter (ICS-10lC) which was dcsigned for X-rays were in good agreement with those of thc Victoreen r-meter, whereas the readings of the ionization chamber type surveymeter made for γ-rays indicated about one half of those of the Victoreen r-meter. The shielding effects of several materials such as polyethylen, rubber, cotton cloth, etc. were measured. Among them, vinyl sheet had the greatest shielding effect.
Survery technique and assessment of absorbed dose are described in appendix.

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The impact of microwave drying and binders (copolyvidone and povidone) on the degradation of acetylsalicylic acid (ASA) and physical properties of granules were compared with conventional drying methods. Moist granules containing ASA were prepared using a high shear granulator and dried with hot air oven, fluid bed or microwave (static or dynamic bed) dryers. Percent ASA degradation, size and size distribution, friability and flow properties of the granules were determined. Granules dried with the dynamic bed microwave dryer showed the least amount of ASA degradation, followed by fluid bed dryer, static bed microwave oven and hot air oven. The use of microwave drying with a static granular bed adversely affected ASA degradation and drying capability. Dynamic bed microwave dryer had the highest drying capability followed by fluid bed, static bed microwave dryer and conventional hot air oven. The intensity of microwave did not affect ASA degradation, size distribution, friability and flow properties of the granules. Mixing/agitating of granules during drying affected the granular physical properties studied. Copolyvidone resulted in lower amount of granular residual moisture content and ASA degradation on storage than povidone, especially for static bed microwave drying. In conclusion, microwave drying technology has been shown to be a promising alternative for drying granules containing a moisture-sensitive drug.

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Hydrogen sulfide (H₂S) has been identified as a novel gasotransmitter and a substantial antioxidant that can activate various cellular targets to regulate physiological and pathological processes in mammals. However, under physiological conditions, it remains unclear whether it is involved in regulating cardiomyocyte (CM) proliferation during postnatal development in mice. This study mainly aimed to evaluate the role of H₂S in postnatal CM proliferation and its regulating molecular mechanisms. We found that sodium hydrosulfide (NaHS, the most widely used H₂S donor, 50-200 μM) increased neonatal mouse primary CM proliferation in a dose-dependent manner in vitro. Consistently, exogenous administration of H₂S also promoted CM proliferation and increased the total number of CMs at postnatal 7 and 14 days in vivo. Moreover, we observed that the protein expression of SIRT1 was significantly upregulated after NaHS treatment. Inhibition of SIRT1 with EX-527 or si-SIRT1 decreased CM proliferation, while enhancement of the activation of SIRT1 with SRT1720 promoted CM proliferation. Meanwhile, pharmacological and genetic blocking of SIRT1 repressed the effect of NaHS on CM proliferation. Taken together, these results reveal that H₂S plays a promotional role in proliferation of CMs in vivo and in vitro and SIRT1 is required for H₂S-mediated CM proliferation, which indicates that H₂S may be a potential modulator for heart development in postnatal time window.

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