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Recombinant Human Tyrosine Hydroxylase Types 1-4 Show Regulatory Kinetic Properties for the Natural (6R)-Tetrahydrobiopterin Cofactor

Shamima Nasrin, Hiroshi Ichinose, Hiroyoshi Hidaka, Toshiharu Nagatsut

The Journal of Biochemistry
1994年 116 巻 2 号 393-398
発行日: 1994年
公開日: 2008/11/18

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Human tyrosine hydroxylase (
hTH
) exists in four isoforms. The four recombinant
hTH
isoenzymes types 1-4 (
hTH
1-4) produced in and purified from Escherichia coli showed regulatory kinetic properties for the natural (6R)-L-erythro-tetrahydrobiopterin (RBPH4) as a cofactor. In contrast, the unnatural cofactor (6S)-L-erythro-tetrahydrobiopterin (SBPH4) and a synthetic cofactor (6RS)-methyl-tetrahydropterin (6MPH4) showed usual kinetic characteristics with each of
hTH
1-4. Substrate inhibition by tyrosine was observed for each of
hTH
1-4 with natural RBPH4. Two different K_m values for pterin cofactor were observed at a high concentration (200 μM) of L-tyrosine only with natural RBPH4, in contrast to a single K_m value for unnatural SBPH4 or synthetic 6MPH4. The present results suggest that in the presence of relatively high concentrations (approximately 100 μM) of tyrosine in vivo, RBPH4 cofactor may have a regulatory role for the activity of all four human isoenzymes in vivo. We also found that recombinant
hTH
1 and 3 were more unstable than
hTH
2 and 4, suggesting that the 4-amino-acid insertion in
hTH
2 and 4 may be responsible for the relative stability of
hTH
2 and 4 isoenzymes.
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Different Coronary Blood Flow Increase in Left Ventricular Hypertrophy Due to Hypertension Compared to Hypertrophic Cardiomyopathy at Elevated Heart Rate

Shigeru TAKECHI, Akikazu NOMURA, Maiko MACHIDA, Tetsuya FUJIMOTO, Shigeo KAKINOKI, Kaoru KOMURO, Akira KITABATAKE

Hypertension Research
2003年 26 巻 10 号 789-793
発行日: 2003年
公開日: 2004/05/25

DOI https://doi.org/10.1291/hypres.26.789

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Coronary vasodilator reserve (CVR) is reduced in patients with left ventricular hypertrophy (LVH). However, it is not clear whether there is any difference between the coronary blood flow increase in LVH caused by hypertension (
HTH
) and that caused by hypertrophic cardiomyopathy (HCM) when the heart rate increases. In this study, 16 subjects with
HTH
, 10 subjects with HCM, and 10 subjects with normotension (NT) were investigated. Average peak velocities at rest, at pacing, and at dilatation were measured using a Doppler catheter placed at the left descending coronary artery to calculate coronary blood flow (CBF) and CVR. CVR at rest was identical in the
HTH
and HCM groups, and in both cases was lower than the resting CVR in NT subjects. There were significant differences in the CVR values at a pacing rate of 120 beats/min among the groups. These values were lowest in HCM, highest in NT, and intermediary in
HTH
subjects. And the percent increase in CBF in HCM at that pacing rate was higher than that in
HTH
(p <0.05) or NT (p <0.05). There was no difference in the percent increase in CBF at this pacing rate between the
HTH
and NT groups. The effects of elevated heart rate on the percent increase in CBF were different between the
HTH
and HCM groups. We conclude that cardiac hypertrophy has qualitatively different effects on coronary circulation depending on whether patients have
HTH
or HCM. (Hypertens Res 2003; 26: 789-793)
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RNAiによる14-3-3η proteinのダウンレギュレーションはPC12D細胞内で発現させたチロシン水酸化酵素の安定性を上昇させる

*中島昭, 林宣宏, 金子葉子, 森啓至, Sabban Esther, 永津俊治

日本生理学会大会発表要旨集
2008年 2008 巻 2P-G-145
発行日: 2008年
公開日: 2008/04/03

DOI https://doi.org/10.14849/psjproc.2008.0.153.1

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The key portion of the N-terminus of human tyrosine hydroxylase type 1 (
hTH
1) that affects the stability of the molecule in mammalian cells has not been clarified except for the observation that the sequence up to Ala⁵² is involved. Therefore, this study was performed to map more precisely the N-terminal sequence that predominantly contributes to the stability of
hTH
1. The results obtained by using N-terminus-deleted
hTH
1 mutants identified the sequence up to Ala²³ as exerting the effect. Next, in order to clarify the effect, PC12D cells exogenously expressing wild-type
hTH
1 or the mutants were established. Then, 14-3-3η proteins in the cells were down-regulated by using the RNAi method, because 14-3-3η can bind Ser¹⁹-phosphorylated
hTH
1. The down-regulation of 14-3-3η proteins in PC12D cells exogenously expressing
hTH
1 enhanced the stability of the wild-type enzyme and that of the mutant lacking the N-terminus up to Ala²³. However, the stability of the mutant was reduced compared to that of the wild-type enzyme. The stability of the mutant with the N-terminus deleted up to Glu⁴³ was not affected by the down-regulation of 14-3-3η. These results suggest that the 14-3-3η protein regulates
hTH
1 stability by acting on the N-terminus up to Ala²³. [J Physiol Sci. 2008;58 Suppl:S153]
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チロシン水酸化酵素のN端に存在するPESTモチーフは本酵素の細胞内安定性に影響を与える

*中島昭, 金子葉子, 森啓至, 永津俊治, 太田明

日本生理学会大会発表要旨集
2007年 2007 巻 1PIP-048
発行日: 2007年
公開日: 2007/07/10

DOI https://doi.org/10.14849/psjproc.2007.0.143.1

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We recently reported that the high intracellular stability of the N-terminus-deleted human tyrosine hydroxylase (TH) type 1 mutants might be generated by the loss of a PEST (proline, glutamate/aspartate, serine and threonine) motif located in the N-terminus sequence of Met¹-Lys¹². However, it is not clear whether only the PEST motif can affect the intracellular stability of human TH type 1 (
hTH
1) proteins. This study was performed to confirm a role of the PEST motif on the stability of
hTH
1. Wild-type
hTH
1 and the 6 mutants (del-12, del-22, del-32 and del-42, in which the first 12, 22, 32 and 42 amino acid residues deleted, respectively; del-Pro2, the Pro² residue deleted; del-Ala11, the Ala¹¹ residue deleted) were expressed in AtT-20 mouse neuroendocrine cells in order to clarify how deeply the PEST motif is involved in the intracellular stability of
hTH
1 protein. The western blot analyses revealed that the mutants del-22, del-32, del-42 and del-Ala11 were much more stable than wild-type in the cells. These results indicate that the PEST motif is critical in regulating the intracellular stability of
hTH
1 protein. Moreover, it has been reported that the phosphorylations of TH at serine residues, Ser¹⁹, Ser³¹ and/or Ser⁴⁰ induce the change in the tertiary structure of the N-terminus of TH protein. Therefore, in this report, we also suggest that the PEST motif and the phosphorylations can affect the intracellular stability of
hTH
1 protein, synergistically. [J Physiol Sci. 2007;57 Suppl:S143]
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High Titer Retroviral Gene Transduction to Neural Progenitor Cells for Establishment of Donor Cells for Neural Transplantation to Parkinsonian Model Rats

Yuji KODAMA, Hideki HIDA, Cha-Gyun JUNG, Hiroko BABA, Mitsuo ISONO, Hidenori KOBAYASHI, Hitoo NISHINO

Neurologia medico-chirurgica
2004年 44 巻 7 号 344-352
発行日: 2004年
公開日: 2005/02/10

DOI https://doi.org/10.2176/nmc.44.344

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Neural progenitor cells (NPCs) are expected to be useful donor sources for cell transplantation therapy in Parkinson's disease. However, control of the differentiational lineage, especially into dopaminergic neurons, is still difficult. Thus, genetic modification of NPCs to produce l-dopa is potentially useful. The present study prepared high titer retrovirus carrying human tyrosine hydroxylase-1 (
HTH
-1) gene.
HTH
-1 gene could be efficiently transduced into NPCs obtained from the E12.5 rat mesencephalon. This retroviral gene transduction caused no apparent changes in survival, proliferation, or differentiation. In vitro,
HTH
-1 gene-transduced NPCs released little l-dopa and addition of tetrahydrobiopterin, the cofactor of tyrosine hydroxylase, was required for production of l-dopa. In vivo, three of seven hemi-parkinsonian model rats that received
HTH
-1 gene-transduced donor NPCs achieved functional recovery. High titer retroviral vector for gene transduction could be used to prepare NPCs for transplantation to hemi-parkinsonian model rats. However, functional recovery after transplantation of
HTH
-1 gene-transduced NPCs was incomplete.

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チロシン水酸化酵素のSer⁴⁰のリン酸化は本酵素の細胞内安定性に影響を与えない

*中島昭, 金子葉子, 森啓至, 永津俊治, 太田明

日本生理学会大会発表要旨集
2006年 2006 巻 3P3-152
発行日: 2006年
公開日: 2007/07/10

DOI https://doi.org/10.14849/psjproc.2006.0_211_2

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It is well established that the phosphorylation of tyrosine hydroxylase (TH) at Ser⁴⁰ is critical in regulating the catalytic activity of the enzyme. However, the influence of the phosphorylation of Ser⁴⁰ on the intracellular stability of TH protein has not been investigated. This study was performed to estimate such a possibility. Although the treatment of rat pheochromocytoma cell line PC-12 cells with forskolin increased the amount of TH phosphorylated at Ser⁴⁰ in the cells, it did not affect the total amount of TH in the cells. Next, human TH type 1 (
hTH
1) of wild-type and a mutant missing the first 52 amino acid residues were expressed as histidine-tagged forms in PC-12 cells, and then the cells were treated with forskolin. However, the phosphorylation of
hTH
1 at Ser⁴⁰ did not affect the amount of the wild-type
hTH
1 protein present in PC-12 cells. Finally, wild-type and a mutant Ser⁴⁰ of which was replaced by Asp (S40D, a mimic of TH phosphorylated at Ser⁴⁰) were expressed in PC-12 cells as histidine-tagged forms or untagged forms. Neither histidine-tagged nor untagged forms showed any difference in their amounts of wild-type
hTH
1 and S40D
hTH
1 present in the cells. Collectively, these results indicate the fact that the phosphorylation of Ser⁴⁰ does not affect the stability of TH protein in PC-12 cells. However, we still do not deny the possibility that the successive phosphorylation of Ser¹⁹ and Ser⁴⁰ may affect the intracellular stability of TH protein. [J Physiol Sci. 2006;56 Suppl:S211]
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チロシン水酸化酵素の細胞内安定性

中島昭, 林宣宏, 金子葉子, 森啓至, 江草悠美, 永津俊治, 太田明

日本生理学会大会発表要旨集
2005年 2005 巻 3P090
発行日: 2005年
公開日: 2005/04/16

DOI https://doi.org/10.14849/psjproc.2005.0.S202.1

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Wild-type human tyrosine hydroxylase (TH) type 1 and 4 mutants (del-52, the first 52 amino acid residues deleted; del-157, the first 157 amino acid residues deleted; RR-EE, Arg³⁷-Arg³⁸ replaced by Glu³⁷-Glu³⁸; and S40D, Ser⁴⁰ replaced by Asp⁴⁰) were expressed in AtT-20 mouse neuroendocrine cells in order to clarify how deeply the N-terminus of TH is involved in the efficient production of dopamine (DA) in mammalian cells. The amounts of DA that accumulated in AtT-20 cells expressing these human TH type 1 (
hTH
1) phenotypes were in the following order: del-52 = del-157 = RR-EE > S40D > wild-type, although the enzyme activities of del-52 and del-157 were lower than those of wild-type, RR-EE, and S40D. The observation on immunoblot analyses that the N-terminus-deleted
hTH
1 mutants were much more stable than wild-type can reconcile the discrepant results. Computer-assisted analysis of the spatial configuration of
hTH
1 identified 5 newly recognized PEST motifs, one of which was located in the N-terminus sequence of Met1-Lys12 and predicted that deletion of the N-terminus region would alter the secondary structure within the catalytic domain. We report that the high stability of the N-terminus-deleted
hTH
1 mutants can be generated by the loss of a PEST motif in their N-termini and the structural change in the catalytic domain, which would promise an efficient production of DA in mammalian cells expressing N-terminus deleted
hTH
1. [Jpn J Physiol 55 Suppl:S202 (2005)]
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Spin Axis Reorientation Transition in Canted Antiferromagnet: Mn(HCOO)₂·2H₂O

Yoshitami Ajiro

Journal of the Physical Society of Japan
1969年 27 巻 4 号 829-839
発行日: 1969/10/05
公開日: 2007/06/01

DOI https://doi.org/10.1143/JPSJ.27.829

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By the measurement of AC magnetic susceptibility, the field induced reorientation transition in Mn(HCOO)₂·2H₂O was observed over the temperature range from 1.3°K to 3.7°K(T_N). The transition could be verified by the change of the angular pattern of proton resonance. The threshold field,
H_th
, was found to be proportional to |T−T_R|^1⁄2 near the spontaneous reorientation transition temperature, T_R(1.7°K). The temperature dependence of
H_th
could be accounted for by the relation
H_th
=\sqrt2H_E|H_A| considering the competing two sorts of anisotropy energies which have different temperature dependences. The angular dependence of
H_th
on the external field direction was given by a hyperbolic relation in the b–d plane and by an elliptic one in the a–c plane. This dependence is discussed phenomenologically.
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New Elementary Excitations in Normal Metals in Ultraintense Magnetic Fields

Tadao Ishii

Journal of the Physical Society of Japan
1975年 38 巻 4 号 954-964
発行日: 1975/04/15
公開日: 2007/06/01

DOI https://doi.org/10.1143/JPSJ.38.954

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A bare-ion Larmor frequency in an applied magnetic field is usually washed out by the electron screening effect to result in an elementary excitation, “phonon·Alfvon”, while only in the “ ultraintense” field whose energy density is of the order of Fermi energy do we have a new excitation, “charged phonon”, besides the phonon·Alfvon.
The charged phonons appear at the threshold field
H_th
determined nearly by
H_th²⁄8π
=ρv_s²⁄2 where v_s is the sound velocity and ρ is the mass density. The Alfvon part predominates the phonon part for the fields stronger than
H_th
(“phonon-vanishing effect”). The threshold field for these phenomena turns out 0.378 MOe for potassium.
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浸セキ塗装における円筒の膜厚

高橋俊郎, 長野年宏, 中村勝彦

金属表面技術
1975年 26 巻 9 号 402-405
発行日: 1975/09/01
公開日: 2009/10/30

DOI https://doi.org/10.4139/sfj1950.26.402

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The author investigated the film thicknesses of two species of water soluble acrylic resin paints, Clear and White, adhered to an aluminum cylinder by dip-coating under stationay conditions. The coatings were made on aluminum wires and round bars under the following conditions. Whithdrawal speed: Clear 0.75, 1.75cm/sec, White 0.52, 1.50cm/sec, Radii of test pieces (cylinders): 6 values of R=0.0625-0.5cm, Ca number: 5.11×10^-3-14.7×10^-3, Go number: 0.2-2.0, The following results were obtained from the above experiments. (1) The theoretical values of film thickness adhered to cylindrical test pieces, reported by D.A. White and J.A. Tallmadge, were in good agreement with these experimental results, (2) The theoretical value of film thickness adhered to cylinders (h′_th)could be determined by multiplying the value of theoretical film thickness adhered to plates (
h_th
) by a coefficient, C_m
h′_th=C_m⋅
h_th
where C_m=1/2.4G_o^0.85/1+2.4G_o^0.85+0.5/G_oG_o=R(ρg/2σ)^1/2 (3) The relation between h′_th and h′_ov (the measured value of film thickness adhered to the cylinders) was identical with the relation between
h_th
and h_ov (the measured value of film thickness adhered to plates). The residual coefficients were approximately equal in both plates and cylinders when the coating were made under the same conditions:
h′_ov=K⋅h′_th=K⋅C_m⋅
h_th

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DIRECT EFFECT OF THYROID HORMONE ON LEFT VENTRICULAR MYOCARDIAL RELAXATION

YASUNOBU DAZAI, ICHIJIRO KATOH, RYOICHI YOSHIDA

JAPANESE CIRCULATION JOURNAL
1992年 56 巻 4 号 334-342
発行日: 1992/04/20
公開日: 2008/04/14

DOI https://doi.org/10.1253/jcj.56.334

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The left ventricular diastolic indices of the hyperthyroid (
HTH
) patients without cardiac disease (n=31) were compared with those of the normal controls (NC) (n=49) using echocardiography. The
HTH
group had a significantly shorter IIa-mitral valve opening time (IIa-MVO), and a significantly larger maximum descending rate of the left ventricular posterior wall (maxPWDR), peak E and peak A than the NC group. IIa-MVO, maxPWDR, peak E and E/A showed significant simple correlation with T₃ in the
HTH
group. To elucidate spurious correlation among these indices and T₃, partial correlation analysis among these indices and its influencing factors were calculated. IIa-MVO, maxPWDR and peak E showed significant partial correlation coefficients with T₃, but peak E had a lesser partial correlation with T₃ than with age and left ventricular end-diastolic volume. Fourteen of the hyperthyroid patients were reexamined after antithyroid treatment. Their diastolic indices were found to be normalized compared with pre-treatment values and showed no significant difference with those of NC group. Augmented myocardial relaxation in patients with hyperthyroidism correlated with the thyroid hormone level, and IIa-MVO and maxPWDR were more effective indices of left ventricular myocardial relaxation than peak E and E/A in hyperthyroidism.
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母斑及び悪性黒色腫に就いての自分の考え

H.Th
. Schreus

日本皮膚科学会雑誌
1962年 72 巻 2 号 109-
発行日: 1962年
公開日: 2014/08/29

DOI https://doi.org/10.14924/dermatol.72.109

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母斑及び悪性黒色腫に関する極めて多数の文献について綜説することは略して，自分が3年来展開して来た新しい見解について主として述べたい.70年前Unnaが滴落説を唱えたか，この説は其後批判もされまた改変もされたが，根本的にはMasson学説の中にも生きて居る. Massonは母斑組織深層にある細胞がSchwann細胞に由来することを発見，母斑組織は表皮基底層に由来するメラノサイトと真皮のSchwann細胞との両方から構成されるものと考えて居る．　自分は，滴落過程の猶お痕跡的に過ぎないであろう乳児期に於て皮膚剥削術を行うことに依って，母斑を確実に治療することを期待した．然し乍ら期待に反して再発を見た．此の再発は母斑組織の深部にあるSchwann細胞に由来するメラノサイトに依るものと考えられる．猶お此のような治療の結果悪性変化か生ずることはないが，時にjuvenile melanoma様の組織像を見ることがある．　自分は悪性黒色腫を新らしい観点に立って考察することを試みて居る．即ち悪性黒色腫は，その病理解剖的，臨床的並びに生物学的の諸性状に於て，悪性腫瘍の常と異るものがあるから、「悪性黒色腫は転移性を有する母斑である．その悪性度は身体の防衛因子に依って規定されるものであって黒色腫細胞に依って規定されるものではない」と考えたい．此の防衛因子を実験的に確証する方法にっいては遺憾乍ら述べられないか，半年以内に死亡すると思われるような軽移を有する悪性黒色腫患者に、抗黒色腫因子を含有するであろう所の正常人の血液を少量反覆輸血することによって，その生命を延長し得たと信ずべき例のあることを報告したい．
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Reliability of Sn–8 mass%Zn–3 mass%Bi Lead-Free Solder and Zn Behavior

Sun-Yun Cho, Young-Woo Lee, Kyoo-Seok Kim, Young-Jun Moon, Ji-Won. Lee, Hyun-Joo Han, Mi-Jin Kim, Jae-Pil Jung

MATERIALS TRANSACTIONS
2005年 46 巻 11 号 2322-2328
発行日: 2005年
公開日: 2005/11/15

DOI https://doi.org/10.2320/matertrans.46.2322

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In order to evaluate the reliability of Sn–8 mass%Zn–3 mass%Bi (hereafter, Sn–8Zn–3Bi) solder, thermal shock tests (temperature range: from 233 to 353 K) and high temperature humidity tests (test condition: 353 K, 85% relative humidity) were conducted. The PCB (Printed Circuit Board) pads were finished by OSP (Organic Solderability Preservative), Sn and Ni/Au. The electric part for test was QFP (Quad Flat Package), and the lead was plated by Sn–3 mass%Bi. The joint strength was estimated by pull testing, and Zn-phase behavior in solder joint was examined.
The pull strengths of Sn–8Zn–3Bi joints in the as-soldered state were measured to be about 16N, irrespective of the PCB pad coatings. After thermal shock testing (hereafter, TS test) up to 1000 cycles, the pull strengths decreased to around 14N. Meanwhile, after high temperature humidity testing (hereafter,
HTH
test) up to 1000 h, the pull strengths deceased to around 6N, irrespective of the PCB pad coatings. The reason for the drastic decrease of pull strength after the
HTH
test was proven to be a crack that was observed along the zinc oxide-phase. The Zn-phase in the Sn–8Zn–3Bi solder changed from a separate acicular shape to a lined-up branched arrangement after
HTH
tests of 1000 h.
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浸セキ塗装における定常状態の膜厚

浸セキ塗装法に関する研究 (第1報)

高橋俊郎, 長野年宏, 中村勝彦

金属表面技術
1973年 24 巻 11 号 631-635
発行日: 1973/11/01
公開日: 2009/10/30

DOI https://doi.org/10.4139/sfj1950.24.631

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A dip coating equipment was prepared for the development of a coating method with water soluble acrylic resin paints on aluminum anodic oxide film. Experiments were conducted by this equipment under the following conditions:
Withdrawing speed V_w=0.33-1.67cm/sec., Density of paints ρ=1.00-1.29g/cm³,
Viscosity of paints μ=0.19-0.66 poise, Surface tension of paints σ=32.0-36.4dyne/cm.
The following results were obtained in the range of 10^-3-10^-1 of capillary number. (1) The the-oretical thickness of dip-coated film (
h_th
) was in good agreement with the following equation of Landau and Levich:
h_th
=0.944(μV_w/σ)^1/6(μV_w/ρg)^1/2 (2) The thickness of film adhered (h_ov) was expressed by the following equation: h_ov=K.
h_th
where K is a residual coefficient, which is 0.70-0.84. (3) The film adhered to a vertical plate became thinner with the lapse of time by “running” of the paint, and the “running was continued until the setting of paint occurred by evaporation. The time for setting seemed to be 500-600sec. in these experiments. (4) The residual coefficient of paints used in the experiments was nearly proportional to the value of μ/ρ. (5) The coated surface of test piece showed avery high degree of smoothness. Therefore, the dip coating would be effective in practical application.
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水平 (外側) 半規管型BPPVクプラ結石症に対する新規治療法

—側方頭部傾斜・跳躍運動によるクプラ結石遊離の試み—

山中敏彰, 澤井八千代, 村井孝行, 藤田信哉, 細井裕司

Equilibrium Research
2010年 69 巻 3 号 127-133
発行日: 2010年
公開日: 2010/08/01

DOI https://doi.org/10.3757/jser.69.127

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This study was performed to determine if a new treatment was effective for cases with benign paroxysmal positional vertigo (BPPV) suggesting cupulolithiasis of the horizontal semicircular canal as characterized by apogeotropic direction-changing nystagmus. We describe herein our head-tilt hopping (
HtH
) exercise designed to release and move otoconia adhesive to the cupula. Subjects were trained to hop with their heads tilted laterally. They completed 3-5 training sessions a day over a 4-week period. Each session ended with a 20-hop trial.
HtH
exercises were performed in 14 cases with intractable horizontal canal BPPV exhibiting persistent nystagmus beating toward the uppermost ear. The spine roll test was tested on all subjects before and immediately after the first trial, as well as after 1 and 4 weeks of the training to evaluate the effect of this treatment on the apogeotropic nystagmus. The nystagmus disappeared or decreased immediately after the first trial with the exercise in 3 and 2 of 14 cases, respectively. The number of subjects showing improvement as assessed by the disappearance or decrease of the nystagmus were in 9 (64.3%) and 11 (78.6%) of all cases tested at 1 and 4 weeks' time, respectively. However the remaining 3 subjects were not affected by this treatment program after 4 week of the training. These results suggest that
HtH
exercises based on the concepts of release of otoconia from the cupula would appear to be feasible as a new therapy for cupulolithiasis associated with intractable horizontal canal BPPV
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浸セキ塗装におけるコーナー部の膜厚

高橋俊郎, 中村勝彦

金属表面技術
1976年 27 巻 3 号 140-144
発行日: 1976/03/01
公開日: 2009/10/30

DOI https://doi.org/10.4139/sfj1950.27.140

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Film thickness distribution of water soluble acrylic resin paints dip-coated on extruded aluminum shapes has been investigated, under stationary state, with some theoretical considerations. Test pieces were of bent plates having curved corners, radii of which varied with each specimen over the range of 0.02-0.2cm. Dip-coating was done with withdrawal speeds of 1.03 and 1.50cm/s. Values of Ca (Ca=μVw/σ) were 4.8×10^-3 and 7.0×10^-3, respectively, and values of Go (Go=R(ρg/2σ)1/2) were in the range of 0.08-0.79, where μ is viscosity, σ is surface tension, ρ is density of paint and g is gravitational acceleration. The following results were obtained by the above experiment. 1) A gradual decrease of film thickness from the plane portion to the corner was observed with the minimum value at the apex. 2) Film thickness at the corner (h″_th) can be caluculated theoretically by multiplying the thickness on plane portion (
h_th
) by a coefficient C′m, that is
h″th=C′
m·hth
=C′m[0.944Ca1/6(μVw/ρg)1/2] where C′m=1/2.4(Go-0.15)^0.85/1+2.4(Go-0.15)^0.85+0.5/(Go-0.15) Go=R(ρg/2σ)^1/2≥0.15
3) Residual coefficient (K) was found to be almost equal both at the bent portion and on the other flat surfaces if the painting conditions would be the same.
h″_ov=K·h″_th=K·C′
m·h_th

whereh″_ov is the measured value.
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基盤入力動に基づく福井平野の地盤構造の推定

*小嶋啓介, 青柳友丈

地盤工学研究発表会発表講演集
2003年 JGS38 巻 1055
発行日: 2003/03/05
公開日: 2005/06/15

DOI https://doi.org/10.11512/jiban.JGS38.0.2109.0

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強震観測データに基づいて,地盤のせん断波速度,層厚,減衰定数などを推定しようとする場合,鉛直1次元地盤構造の推定に限定しても,複数の地震計が異なる深さに設置されていることが望ましい.しかしながら実際に利用できる地震計は,気象庁の計測震度計やK-net観測網のように表層の3成分計が圧倒的に多い.ここでは,基盤入力動が観測された点と,表層震動のみが記録され地盤構造を推定しようとする点を一組とし,両者の基盤入力動が等しくなるように,推定点の層厚やせん断波速度などを最適化することにより,地盤構造の同定する方法を福井平野の観測地震動に適用した結果を報告する.
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27p-PS-30 3次元DIMER STATISTICSの厳密解---2×3×n格子

鳴海英之, 細矢治夫

年会講演予稿集
1992年 47.3 巻 27p-PS-30
発行日: 1992/03/12
公開日: 2018/03/27

DOI https://doi.org/10.11316/jpsgaiyod.47.3.0_400_2

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超音波振動旋削(第2報)

隈部淳一郎

精密機械
1960年 26 巻 303 号 206-210
発行日: 1960/04/05
公開日: 2009/06/30

DOI https://doi.org/10.2493/jjspe1933.26.206

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In this report, the surface roughness produced by means of ultrasonic turning is discussed.
According to the machining tests of aluminum, brass, copper and mild steel, which have been successfully conducted by using the improved tool post, it is found that the surface roughness obtained by this method is very close to the theoretical roughness at the lower cutting speed than the critical one V_c=0.376 af m/mn (a amplitude, micron: f=frequency, kilocycle/s).
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溶液中でタンパク質のコンフォメーション変化を観る(B・物理系薬学)

毛塚雄一郎

ファルマシア
2004年 40 巻 8 号 761-762
発行日: 2004/08/01
公開日: 2018/08/26

DOI https://doi.org/10.14894/faruawpsj.40.8_761

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