Metabolism of body fluids and electrolytes which related not only to aldosterone alone but to other corticosteroids, for example, glucocorticoids had mineral action. And the simultaneous administration of aldosterone and corticosterone consistently resulted in a greater potassium diuresis than did the aldosterone alone.
Therefore, body fluids and electrolytes are aummation of many corticosteroids which had biological action, and can't know by the determination of simple corticosteroids.
The author report on the systemic determination of aldosterone, Comp. , Comp. , Comp. , and the newly separated contaminants.
Method :
The 24 hours urine specimen is pooled at °C and acidified to pH 1. with conc. HCL, then standing 24 hours at 15°C.
Urine was extracted by chloroform and dried. Extract was treated with Florisil column chromatographies and 4 consecutered and different paper chromatographies with Chloroform-Formamide System, Bush-C System, System, and Bush- System, to separated corticosteroid partitions.
Results :
1. Recoveries of mg/100 ml H₂O standard samples after times continued extraction by .1 volume chloroform, for Comp. , Comp. , and DOC is 95%, and Comp. is %.
2. Additional chloroform re-extraction, Comp. , Comp. , and DOG do not appear in the 7th chloroform extraction, but Comp. appear in the amount of 60μg in th chloroform and th extraction is nothing, thus this determination necessitate at least consecutered extractions.
. Comp. , Comp. , and Comp. appear in 25% methanol in chloroform fraction by the use of the Florisil column chromatography is made, and recovery is about 100% in condition of 60 drops/minutes.
4. Separate Comp. III, Comp. V, Comp. VI, and Comp. VII from aldosterone partition in Neher-Wettstein's method.
. New contaminants were discover from Comp. and Comp. partition after Bush-C System paper chromatography, called Comp. -, Comp. ; and, other conatminants named M₁- Z₁-, HC₁-, AC₁ and AC₂ in Chloroform-Formamide and Bush-C System paper chromatography.
. Examination of the purities of Comp. , Comp. , Comp. , and aldosterone partitions is made use of the ultra violet absorption spectrum, and necessitates 4 (C-→Bush-C→→Bush-), or (C-→→Bush-), paper chromatographies to satisfactorily separate these compounds.
7. The H₂SO₄ chromogen spectrum of these partition correspond with the standard compounds.

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[1] The purpose of this reserch is to make clear what type of design can give good effects to win the high readership score. At the begining I had to know what factors decide the readership score, and how to change the quality of the desing into numerical value. I have used the method of multi-dimentional analysis with the help of a computer, and got the estimation formula, with 7 kinds of factors, as follows ; A^^〜=+C+D+K+aE+bF+cG. A^^〜=estimated values of the readership score. 1) , C, D, K : these four factors are given as category values (so we call them); each factor is classified into several categories. =week day, classified into 7 categories. C=pages of a newspaper, which are characterized by the kinds of articles, news, and the other reading matters, classified into categories. D=kinds of goods and trades of the advertizement, classified into 14 catedories. K=constants, given to each 13 surveys. 2) , , G : these factors are given as numerical values. G=area of advertizement, measured by the unit of column in the whole page length, being classified into kinds of area (2., ., , 7, 10, 15) and 15 column means a whole page, about 2,000cm^2. , are the marks obtained in the design of advertizements. =sum of the marks obtained in each design element on the advertizement. =the marks obtained on the whole effect of the design. ) a, , c : coefficients Using the above formula with the category values and the coefficients, we can obtain the naked design effect from the actual value A of readership score: aE+bF=A-(+C+D+K+cG). In this research, I used 1,829 data of readership score from 1960 to 1968, being obtained by 13 survevs, spring and autumn twice a year. The sample of size each survey was ,541, 2,251, or 200 in the other 11 surveys. Multiple correlation coefficient between the estimated value A^^〜 and the actual value A is .951. The table 2^* shows the contribution indexes of each factor by kinds of expression-(1) Range : the absolute difference between the maximum and the minimum category values, (2) Standard deviations of the category values, () Partial correlation coefficient : the relationship between the actual values A and each factors. *see the table 2 in the thesis in Japanese. [2] The important point was in the determination of the values of , . is the sum of the marks obtained in each design element, classified into four kinds : =_1+_2+_+_4. design elements [table] _1, _2, _, _4, these values have the grades, as , 1, 2, , 4 and is given to the design that has no attractive effect or no applicable element. And obtain the marks of to grades as the sum of them. The values of have also grades, to 4. The principles to determine the values of , are as follows : a) The marks obtained of , must be the relative values among the each survey, and at the same time, they must have constancy within the same survey-the same elements of design must win the same marks obtained. ) They must be determined as to win the highest multiple correlation coefficient, when they are put into the estimation formula. c) They must be reasonable. In order to justify them, we must carry many researches on the actual condition. The frequency of accurence of each grade of , values, as the table -4, and - in the thesis in Japanese.

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It has been shown after mathematical and mechanical investigation that Limit Equilibrium Method (LEM) can be considered as a method to obtain the necessary condition of Slip Line Method (SLM), and that the solution can be obtained under the condition that F_s=1 and ∂F_s/∂θ=O, where F_s is the safety factor and θ is the inclination of the plane on which the safety factor is defined. Next, the generalized LEM (GLEM) is proposed, dealing with the following points : (1) Triangular or quadrangular blocks can be treated; (2) Safety factors are defined also on interblock planes; and () All types of plastic problems, slope stability, bearing capacity, and earth pressure are identically formulated. Two situations regarding treatment of the moment equilibrium condition are discussed. GLEM is applied to well-known problems. The results agree well with those obtained by theoretical methods. GLEM can be used to obtain the distribution of earth pressure or bearing capacity. GLEM, Iike ordinary LEM, is very effective for the practical problems, but GLEM is more theoretical and it can result in more accurate solutions.

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Recently we saw two patients with acute hepatitis, infected with hepatitis virus (HEV) of a rare genotype . One patient (Tokyo/2012) had traveled Spain 1 month before developing hepatitis, while the other patient (Saitama/2014) did not have history of traveling abroad. Nearly entire genomic sequence of HEV was recovered from both patients; 7,336 nucleotides (nt) from the Tokyo case in 2012 (isolate name=JAO-SpaTok12, accession=LC055972) and 7,306 nt from the Saitama case in 2014 (JMH-Sai14, LC055973). Phylogenetic analysis revealed that JMH-Sai14 from the Saitama patient, who denied having traveled abroad, could be classified into a subgroup of genotype , together with European isolates as well as an already reported Japanese isolate HE-JA12-0725 (850879) from a patient living in Gunma and having never traveled abroad when he/she developed hepatitis in 2012. On the other hand, the Tokyo patient's sequence segregated to a separate branch together with European isolates. Based on these findings it is possible that HEV genotype has already made inroad into Japan as in the case of .

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Six new cembranoids sarcophytol P (), R (4), S (), K (), (11), and T (13) were isolated from the soft coral Sarcophyton glaucum. Sarcophytol P () was shown to be the 20-hydroxy derivative of the major component sarcophytol A (1), and afforded the cyclization product in CHCl_ at room temperature, in a same way as in 1. Sarcophytols R (4) and S () were correlated to 1, by conversion of its 7R,R and 7S,S epoxide derivatives. Sarcophytol K() was a 13, 14-dihydroxycembranoid having a 1,Z-diene moiety. The absolute configuration of and its 1Z,- and 1Z,Z-isomers sarcophytol (2) and J (10) were determined by circular dichroism study of their bis-p-dimethylaminobenzoate derivatives. Sarcophytols (11) and T (13) were 1- and 1,Z-isomers of 1. Compound 11 showed characteristic broadening of the ^1H-NMR chemical shifts, due to the restricted conformational interconversion. Using the three cembranoids sarcophytols (11, 1,), N (15, 1Z,Z) and T (13,1,Z), spontaneous autoxidation-cyclization, in CHCl_,was examined, in order to compare the stereochemical course of the reaction with that of 1 (1Z,), which affords trans-fused bicyclo[..]tetradecene systems. The 1,Z-isomer 13 gave the same cyclization product 18, as in the reaction of 1, even though it is isomeric at C-1,. The 1Z,Z isomer 15 gave 19, in consequence of the reversed geometry at C- of 15. The 1,-isomer (11) gave the bicyclic product , having an antipodal fusion as compared with 19. This was confirmed by PCC oxidation of 19 and , giving enantiomeric ketones 23 and 24 respectively. The 1(14)-epoxide 26 was shown to be the immediate precursor of , and acounted for the inversion of the geometry at C-1 of the cyclization product. Compound 26 is isomeric with the epoxide 17 derived from 1. The epoxide 17 is the postulated precursor in the conversion of 13 to 18. It is noteworthy that the mode of antipodal fusion of the cyclopentane rings, in 18 and , was controlled by the chirality of the epoxy rings. The C-14 hydroxyl group participates in the transannular cyclization, but was found not to be the requisite functional group for the reaction. Similar treatment of cembrene C (30), the parent hydrocarbon of 1, also reacted in CHCl_ giving the bicyclic product 32.

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Tokamak D-T power reactor with day-long operation is investigated, where plasma current is driven inductively.Thereby the high power multiplication factor (typically Q>1, 000) is attained easily.For day-long operation only with the use of inductive current drive, the large plasma major radius is necessary to provide sufficient magnetic flux in the Ohmic transformer.Parameters of tokamak reactor for day-long operation are typically major radius 12m, minor radius 1.m with elongation ratio1. and triangularity., toroidal mag netic field12.T, and plasma current .7MA.Plasma volume is comparable to the current-driven reactors, such as SSTR and ARIES in spite of large major radius, because the aspect ratio is relatively high.

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A full account is given of the chemical behavior observed for 7, -dialkyladeninium salts (16). On treatment with boiling 1N aqueous NaOH for 60min, 16a, , d, (X=I), 16c (X=Br), and 16 (X=ClO₄) rearranged to isomeric , 7-dialkyladenines (21a-) in 50-91% yields. Treatment of the salts with .N aqueous at room temperature for 30-min or with Amberlite CG-400 (OH^-) in H₂O at room temperature gave the ring-opened derivatives a- (in the trans-formamide form) in 56-% yields, and rate constants for the ring-opening reactions of 16a, , d-g (X=ClO₄) and 16c (X=Br) leading to a-g were determined in H₂O at pH .84 and ionic strength .50 at 25°C. Cyclization of a with NaH in AcNMe₂ at room temperature or with boiling 1N aqueous NaOH produced 21a in 84% or 72% yield, respectively.In solution, the trans-formamides seemed to transform slowly into the cis-formamides 23, attaining equilibria. The existence of such an equilibrium in D₂O or Me₂SO- at 25°C or in H₂O at pH .84 and ionic strength .50 at 25°C was kinetically confirmed in the case of a, and the mechanism of the rearrangement of 16 to 21 through is discussed on the basis of the above kinetic results and Deslongchamps' theory of stereoelectronic control. On treatment with NaBH₄ in MeOH at room temperature, 16a (X=I) furnished the 7, -dihydro derivative 28 (84% yield), which slowly decomposed in H₂O at 60°C to give a in 49% yield.The 7, -dialkyladeninium salts (16) were found to be obtainable from N'-alkoxy-1-alkyl--formamidoimidazole-4-carboxamidines () through an alternative synthetic route : Alkylations of with alkyl halides in HCONMe₂ in the absence of base, followed by hydrogenolysis of the N'-alkoxy group and cyclizatio (or vice versa) produced 16 in acceptable yields. In order to interpret the proton nuclear magnetic resonance spectrum of a, the 2-deuterated species 26 was also synthesized from 24 via 25 and 27.

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Nアルキル,4:,10-ペリレンテトラカルボン酸モノアンヒドリド=モノイミド[4a～]と芳香族アミン(アニリン,p-トルイジン,p-アニシジン,,-キシリジン,4-アミノナゾベンゼン,およびo-フェニレンジアミン)を縮合して非対称型,4:,10-ペリレンビス(ジカルボキシミド)誘導体-N-アルキル-N'-アリール-,4:,10-ペリレンビス(ジカルボキシミド)(〔a～〕,〔a～〕,〔7a～〕,〔a～〕,〔a～〕,および〔10a～〕)を合成した. これらの各誘導体はすべて赤色系の色相を示し, 顔料試験の結果N-ブチル-N'-アリール-,4:,10-ペリレンビス(ジカルボキシミド)(たとえば〔〕や〔〕)がとくにすぐれた耐光性を示した.

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This study aimed to determine the prevalence of fecal carriage of extended spectrum β-lactamase (ESBL) and/or plasmidic AmpC β-lactamase (pAmpC) producing Escherichia coli among dogs (n=428) in Turkey. Polymerase chain reaction (PCR) and sequencing were used to characterize genes encoding β-lactamase and plasmid mediated quinolone resistance (PMQR). Antimicrobial susceptibility testing and PCRs for virulence genes and phylogenetic groups were also performed. Cefotaxime resistant

. coli isolates were detected in 95 (.2%) of the swab samples. Sequencing analysis results showed occurrence of various β-lactamase genes: bla_CTX-M-15 (62), blaTEM-

1b

(42), bla_CMY-2 (

22

), blaCTX-M-

3

(16), bla_CTX-M-1 (15), bla_OXA-1 (

9

) and bla_SHV-12 (

3

) alone or in combination. The most frequently encountered phylogenetic group was group A₁ (35.

8

%), followed by group D₂ (

22

.1%),

B

1 (15.

8

%), D₁ (

9

.

5

%),

A₀

(7.4%),

B2₂

(

5

.

3

%) and

B2₃

(4.2%), respectively. PMQR genes, aac(

6

’)-Ib-cr, qnrS1 and qnrB10 were detected in 25.

3

, 10.

5

and 1.1% of the isolates, respectively. While all isolates were susceptible to imipenem and amikacin, resistance rates to non-β-lactam antibiotics ranged from 20.

0

% for tobramycin to 56.

8

% for tetracycline. The virulence genes were only detected in 34 (36.2%) of the isolates and this isolates carried single or various combination of virulence genes of iucD, papC, papE,

f17a

-A and eaeA. Four isolates were identified as human virulent pandemic CTX-M-15 producing

E

. coli clone O25

b

:ST131/

B

2. To the best of our knowledge, this is the first study to show fecal carriage of ESBL/pAmpC type β-lactamase producing

E

. coli isolates among dogs in Turkey.

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A detailed account is given of the syntheses of -alkyl-2-deuterioadenines (-d), adenosine-2-d (), and 2'-deoxyadenosine-2-d () from the -substituted adenines 1- through cyclization of the aminoimidazolecarbox-amidine salts or the corresponding free bases with formic-d acid-d or 1-(formyl-d)-2(1H)-pyridone. Glycosidic hydrolysis of with boiling .N aqueous HCl afforded adenine-2-d (a) in 77% yield. Peracid oxidation of a- produced the corresponding 1-N-oxides (12a-) in fair yields. Methylation of -methyl- (12) and -benzyladenine-2-d 1-oxide (12d) and adenosine-2-d 1-oxide (12) with MeI in AcNMe₂ gave the corresponding 1-methoxy derivatives (13, d, ) in good yields. Dimroth rearrangements of 13, 13d, and 13 via the free bases 14, 14d, and 14 furnished the -methoxy isomers 15, 15d, and 15, but their isotopic purities were unsatisfactory. Unambiguous assignments of the purine-ring proton signals in the proton nuclear magnetic resonance spectra of the unlabeled adenines (1a-, 2a-, , d, and 10) have been made by comparison with those of the labeled species (a-, 12a-, 13, d, and 14).

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Sectional boundedness () and functional sectional convergence (FAK) in FK-spaces (=locally convex, metrizable and complete spaces of complex sequences x = ({x_k})_{k = 1}^∞ ) are considered with emphasis on sums + = \left{ {x:x = a + , a \in , \in } \ ight} of FK-spaces and . Notations and definitions: Let {δ ^k} = (δ _n^k)_{n = 1}^∞, δ _n^n = 1 if n \ k. {P_n}x = ∑\ olimits_{k = 1}^n {{x_k}{δ ^k}} is the n-th section of x. Let be an FK-space, the space of linear and continuous functionals on . Let {P_n}x \in for every n = 1, 2, … . x has in if \left{ {{P_n}x} \ ight} is a bounded set in , x has AK in if {\lim _n}{P_n}x = x in , x has FAK in , if {\lim _n}\left( {{P_n}x} \ ight) exists for every n = 1, 2, …, x has in if x \in {\bar \varphi ^} (=the closure of φ in ), where φ is the space of x with only finitely many {x_k} \ . The spaces {E_AB}, {E_Ak}, {E_FAK}, {E_AD} consisting of all x which have respectively , AK, FAK, in , are FK-spaces with appropriate topologies. Let {} = \left{ {x:{x_k} = ({δ ^k}){ for some } \in '{ if }{δ ^k} \in } \ ight}, {^r} = \left{ {x:{{\sup }_n}\left| {∑\ olimits_{k = 1}^n {{x_k}yk} } \ ight| < ∞ { for every }y \in } \ ight}. If A and are sets of complex sequences, then (A → ) = \left{ {x:({x_k}{y_k}) \in { for every }y \in A} \ ight}.

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エチレングリコールにγ-線照射を行なうと, (a)水素, ()メタン, (c)-酸化炭素, (d)エタン, ()アセトアルデヒド, ()メタノール, (g)エタノール, (h)酢酸, (i)酢酸ヱチル, (j)アセトアルデヒドエチレンアセタール,(k)グリコールモノアセテートおよび(l)水が生成する。これら生成物のG値は,吸収エネルギーとともに,()・()・(h)は不変, ()・(d)・(g)・(i)は増加,(a)・(j)・(l)は減少する。また1.×10²¹ev/ml のエネルギー吸収の際の,それらのG値はつぎのとおりである。G(a)=1., G()=.7×10^-2, G(c)=1.×10^-2, G(d)=.×10^-2, G()=4., G()=1.7, G(g)=2., G(h)=1.1, G(i)=.7, G(j)=48, G(l)= これら生成物のうち, (a)・()・()・()は1次生成物で, 他は2次生成物と推論される。

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