詳細検索結果
以下の条件での結果を表示する: 検索条件を変更
クエリ検索: %22%E7%9F%B3%E5%8E%9F%E9%83%BD%E7%9F%A5%E4%BA%8B%22
5,094,569件中 1-20の結果を表示しています
  • 小田切 孝人, 田代 眞人
    ウイルス
    2013年 63 巻 2 号 233-240
    発行日: 2013/12/25
    公開日: 2014/10/31
    ジャーナル フリー
  • George W. Lee
    燃料協会誌
    1973年 52 巻 3 号 171-177
    発行日: 1973/03/20
    公開日: 2010/06/28
    ジャーナル フリー
  • Grover Loening
    日本航空学会誌
    1963年 11 巻 119 号 373-380
    発行日: 1963年
    公開日: 2009/05/25
    ジャーナル フリー
  • I.
    F
    . Davies
    Journal of Light & Visual Environment
    1984年 8 巻 1 号 1_9-1_20
    発行日: 1984年
    公開日: 2011/05/20
    ジャーナル フリー
  • A.M. BRITTO, C. SAVVIDOU, M.J. GUNN, J.R. BOOKER
    土質工学会論文報告集
    1992年 32 巻 1 号 13-25
    発行日: 1992/03/15
    公開日: 2008/02/29
    ジャーナル フリー
    One method currently being considered for the disposal of high level radio-active waste is burial in the seabed. When a hot object is buried in soil, the temperature of the soil adjacent to the object is raised and there is transient heat flow away from the object. The rise in the temperature of the soil causes excess pore pressures to be generated and transient pore water diffusion also takes place. A finite element formulation of the coupled heat flow and consolidation problem is presented. The computer implementation of this formulation is found to compare favourably with analytical solutions based on the same physical principles.
  • 山本 雅史, Abkenar Asad Asadi, 松本 亮司, 根角 博久, 吉田 俊雄, 國賀 武, 久保 達也, 冨永 茂人
    園芸学会雑誌
    2007年 76 巻 1 号 36-40
    発行日: 2007年
    公開日: 2007/03/06
    ジャーナル フリー
    カンキツ主要12種を用いて染色体のクロモマイシン
    A3
    (CMA) 染色を行った.染色体は CMA(+) バンドの有無および位置から
    7
    種類に区分できた.すなわち,CMA(+) を A: 両端および動原体近傍に有する,
    B
    : 一方の端部と動原体近傍に有する,C: 両端に有する,D: 一方の端部に有する,
    E
    : CMA(+) がない,
    F
    : 動原体近傍に有する,Dst: 付随染色体を有する D 型,である.各種はこれらのうち 2~6 種類の染色体を有し,独自の CMA バンドパターンを示した.C. medica では 2
    B
    +
    8
    D +
    8
    E
    C. limon では 1
    B
    + 1C +
    8
    D +
    8
    E
    C. aurantifolia では 2
    B
    +
    9
    D +
    7
    E
    C. aurantium では 1A + 1
    B
    + 1C +
    7
    D +
    8
    E
    C. sinensis では 2
    B
    + 2C +
    7
    D +
    7
    E
    C. maxima では
    3
    A +
    3
    C +
    4
    D +
    8
    E
    C. paradisi では 2A +
    3
    C + 6D +
    7
    E
    C. ichangensis では 2
    B
    + 2C + 12D + 2
    E
    C. latipes では 2A +
    5
    C +
    8
    D +
    3
    E
    C. micrantha では 1
    B
    + 11D +
    4
    E
    + 2Dst,C. macroptera では 2
    B
    + 1C + 11D +
    3
    E
    + 1
    F
    C. hystrix では
    3
    B
    + 1C +
    8
    D +
    3
    E
    + 2
    F
    + 1Dst であった.
  • 黒川 美貴雄, 吉田 耕治, 永井 保嵩, 宇野 準
    Chemical and Pharmaceutical Bulletin
    1983年 31 巻 12 号 4312-4318
    発行日: 1983/12/25
    公開日: 2008/03/31
    ジャーナル フリー
    Two new partially saturated tricyclic ring systems, 6, 6a,
    7
    ,
    8
    ,
    9
    , 10, 10a, 11-octahydro-11-oxodibenz [
    b
    ,
    e
    ] oxepins (
    3
    a and
    3
    b
    ), and -thiepins (
    4
    a and
    4
    b
    ) were synthesized. Compounds
    4
    a and
    4
    b
    were desulfurized to give a pair of isomeric 2-methylbenzoylcyclohexanes (10a and 10
    b
    ). Deuterated
    4
    a and
    4
    b
    (11a and 11
    b
    ) were prepared starting from butadiene-d6 (12). The stereochemical features of
    3
    a (trans),
    3
    b
    (cis),
    4
    a (trans) and
    4
    b
    (cis) are compared with those of 10a, 10
    b
    , 11a and 11
    b
    on the basis of proton nuclear magnetic resonance data.
  • 琴尾 幸徳, 石川 成実, 田辺 順子, 御園 生尭久
    日本化学会誌(化学と工業化学)
    1980年 1980 巻 9 号 1391-1396
    発行日: 1980/09/10
    公開日: 2011/05/30
    ジャーナル フリー
    Nアルキル
    3
    ,
    4
    :
    9
    ,10-ペリレンテトラカルボン酸モノアンヒドリド=モノイミド[
    4
    a~
    e
    ]と芳香族アミン(アニリン,p-トルイジン,p-アニシジン,
    3
    ,
    5
    -キシリジン,
    4
    -アミノナゾベンゼン,およびo-フェニレンジアミン)を縮合して非対称型
    3
    ,
    4
    :
    9
    ,10-ペリレンビス(ジカルボキシミド)誘導体-N-アルキル-N'-アリール-
    3
    ,
    4
    :
    9
    ,10-ペリレンビス(ジカルボキシミド)(〔
    5
    a~
    e
    〕,〔6a~
    e
    〕,〔
    7
    a~
    e
    〕,〔
    8
    a~
    e
    〕,〔
    9
    a~
    e
    〕,および〔10a~
    b
    〕)を合成した. これらの各誘導体はすべて赤色系の色相を示し, 顔料試験の結果N-ブチル-N'-アリール-
    3
    ,
    4
    :
    9
    ,10-ペリレンビス(ジカルボキシミド)(たとえば〔
    5
    e
    〕や〔6
    e
    〕)がとくにすぐれた耐光性を示した.
  • 林 雄二, 松本 武
    天然有機化合物討論会講演要旨集
    1981年 24 巻 42
    発行日: 1981/09/10
    公開日: 2017/08/18
    会議録・要旨集 フリー
    Reactions of nor-diterpenoid dilactones isolated from Podocarpus plants have been investigated. The
    7
    :
    8
    ,
    9
    : 11-dienolide type [C] dilactones, the least available but biologically the most active members, are mainly derived from naturally abundant dilactone components of α-pyrone type [A] and
    7
    :
    8
    -epoxy-
    9
    : 11-enolide type [
    B
    ] for total-synthetic and biological purposes. From nagilactone
    E
    (
    5
    ), for example, a combined sequence of hydrogenolysis of
    7
    :
    8
    -epoxide group and dehydration gives nagilactone
    F
    (
    7
    ),
    3
    β-hydroxynagilactone
    F
    (
    8
    ) and 2:
    3
    -dehydronagilactone
    F
    (19), which have been found from natural sources. From nagilactone A (1), photo-hydration and concomitant double bond migration of its acetate results in a lactol (
    22
    ), which is reduced to 1β-hydroxynagilactone
    F
    (
    9
    ), another natural type [C] dilactone. Derivation of the type [A] or [
    B
    ] from others is not successful. Some attempts for conversion of the ring A functional groups have also been studied.
  • Joey
    B
    . Tanney, Leonard J. Hutchison
    Mycoscience
    2012年 53 巻 1 号 31-35
    発行日: 2012年
    公開日: 2023/03/31
    ジャーナル オープンアクセス

    The ability of Climacodon septentrionalis to immobilize and kill a mycophagous nematode (Aphelenchoides sp.) in vitro is described for the first time. Two isolates produced droplets (20–45 μm in diameter) that formed at the apices of tall, stalked, and branching secretory cells (700–1,500 μm tall). On 2% modified malt extract agar, nematodes became enveloped in the droplets, which restricted their ability to move and resulted in complete immobilization and death within several hours of contact. The rate of decomposition of the nematodes varied considerably, with most individuals persisting for weeks whereas others were degraded within several days and appeared to be colonized by dense hyphal growth. This study provides the first documentation of a non-agaricoid fungus producing secretory cells that are able to immobilize nematodes.

  • 菊池 志津子, 御領 政信, 石井 映子, 岡田 幸助
    日本獣医師会雑誌
    1998年 51 巻 2 号 65-69
    発行日: 1998/02/20
    公開日: 2011/06/17
    ジャーナル フリー
    伝染性ファブリキウス嚢病ウイルス (IBDV) による免疫抑制下で, トリレオウイルス (ARV) と大腸菌 (
    E
    . coli
    ) の混合感染による頭部腫脹症候群の再現を試みた. 各群
    8
    9
    羽の1週齢SPF鶏にIBDV993株を経口接種し,
    3
    週齢でARVMJ3810株を点眼接種後1,
    4
    あるいは
    7
    日に大腸菌を点眼接種し, 接種後20日に剖検したところ, 頭部の腫脹はみられなかったが, それぞれ2/
    9
    羽,
    4
    /
    9
    羽, 1/
    8
    羽に頭蓋骨air spaceおよび中耳内に炎症性病変および肉芽腫がみられた. ついで, 別の
    8
    群 (A~H; 各群13~14羽) にはそれぞれ次の組合せで接種を行い, 大腸菌接種後
    4
    ~20日に剖検した: A (無処置),
    B
    (
    E
    . coli
    ), C (ARV), D (ARV+
    E
    . coli
    ),
    E
    (IBDV),
    F
    (IBDV+
    E
    . coli
    ), G (IBDV+ARV), H (IBDV+ARV+
    E
    . coli
    ) では,
    B
    群の2羽, H群の
    3
    羽には軽度の眼瞼腫脹がみられ, 軽度の炎症性病変を含めると, 組織学的には
    B
    群の
    3
    羽, D群の2羽,
    F
    群の1羽, G群の2羽, H群の
    5
    羽に頭部の病変がみられたが, 頭部皮下織炎は
    B
    群の2羽, H群の
    4
    羽のみに認められた.
  • 黒川 美貴雄, 糸川 彰, 松本 純一, 福本 吉久, 月原 冨武
    Chemical and Pharmaceutical Bulletin
    1992年 40 巻 9 号 2270-2274
    発行日: 1992/09/25
    公開日: 2008/03/31
    ジャーナル フリー
    11-Amino-6, 6a,
    7
    ,
    8
    ,
    9
    , 10, 10a, 11-octahydrodibebenzo[
    b
    ,
    e
    ]thiepines (6a-d) and -oxepines (
    7
    a-d) were synthesized by the Leuckart reaction of 6, 6a,
    7
    ,
    8
    ,
    9
    , 10, 10a, 11-octahydro-11-oxodibenzo[
    b
    ,
    e
    ]thiepines (1a,
    b
    )and -oxepines (2a,
    b
    ) followed by hydrolysis of the reaction products
    4
    a-d and
    5
    a-d, respectively. The four diastereomers, cis(6a-H, 10a-H)-cis(10a-H, 11-H) 6a and
    7
    a, cis(6a-H, 10a-H)-trans(10a-H, 11-H) 6
    b
    and
    7
    b
    , trans(6a-H, 10a-H)-trans(10a-H, 11a-H)6c and
    7
    c, and trans(6a-H, 10a-H)-cis(10a-H, 11-H) 6d and
    7
    d, were isolated and their configurations and conformations were elucidated by chemical methods together with 1H-nuclear magnetic resonance spectroscopic and X-ray crystallographic analyses.
  • Özkan ASLANTAŞ, Ebru Şebnem YILMAZ
    Journal of Veterinary Medical Science
    2017年 79 巻 6 号 1024-1030
    発行日: 2017年
    公開日: 2017/06/16
    [早期公開] 公開日: 2017/04/27
    ジャーナル フリー

    This study aimed to determine the prevalence of fecal carriage of extended spectrum β-lactamase (ESBL) and/or plasmidic AmpC β-lactamase (pAmpC) producing Escherichia coli among dogs (n=428) in Turkey. Polymerase chain reaction (PCR) and sequencing were used to characterize genes encoding β-lactamase and plasmid mediated quinolone resistance (PMQR). Antimicrobial susceptibility testing and PCRs for virulence genes and phylogenetic groups were also performed. Cefotaxime resistant

    E
    . coli isolates were detected in 95 (
    22
    .2%) of the swab samples. Sequencing analysis results showed occurrence of various β-lactamase genes: blaCTX-M-15 (62), blaTEM-
    1b
    (42), blaCMY-2 (
    22
    ), blaCTX-M-
    3
    (16), blaCTX-M-1 (15), blaOXA-1 (
    9
    ) and blaSHV-12 (
    3
    ) alone or in combination. The most frequently encountered phylogenetic group was group A1 (35.
    8
    %), followed by group D2 (
    22
    .1%),
    B
    1 (15.
    8
    %), D1 (
    9
    .
    5
    %), A0 (
    7
    .
    4
    %),
    B22
    (
    5
    .
    3
    %) and
    B23
    (
    4
    .2%), respectively. PMQR genes, aac(6’)-Ib-cr, qnrS1 and qnrB10 were detected in 25.
    3
    , 10.
    5
    and 1.1% of the isolates, respectively. While all isolates were susceptible to imipenem and amikacin, resistance rates to non-β-lactam antibiotics ranged from 20.0% for tobramycin to 56.
    8
    % for tetracycline. The virulence genes were only detected in 34 (36.2%) of the isolates and this isolates carried single or various combination of virulence genes of iucD, papC, papE,
    f17a
    -A and eaeA. Four isolates were identified as human virulent pandemic CTX-M-15 producing
    E
    . coli
    clone O25
    b
    :ST131/
    B
    2. To the best of our knowledge, this is the first study to show fecal carriage of ESBL/pAmpC type β-lactamase producing
    E
    . coli
    isolates among dogs in Turkey.

  • 小林 優, 家坂 貴子, 中野 江身子, 平山 耕一郎
    天然有機化合物討論会講演要旨集
    1989年 31 巻 71/P2-1
    発行日: 1989/09/17
    公開日: 2017/08/18
    会議録・要旨集 フリー
    Six new cembranoids sarcophytol P (
    3
    ), R (
    4
    ), S (
    5
    ), K (
    8
    ),
    F
    (11), and T (13) were isolated from the soft coral Sarcophyton glaucum. Sarcophytol P (
    3
    ) was shown to be the 20-hydroxy derivative of the major component sarcophytol A (1), and afforded the cyclization product 6 in CHCl_
    3
    at room temperature, in a same way as in 1. Sarcophytols R (
    4
    ) and S (
    5
    ) were correlated to 1, by conversion of its
    7
    R,
    8
    R and
    7
    S,
    8
    S epoxide derivatives. Sarcophytol K(
    8
    ) was a 13, 14-dihydroxycembranoid having a 1
    E
    ,
    3
    Z-diene moiety. The absolute configuration of
    8
    and its 1Z,
    3
    E
    - and 1Z,
    3
    Z-isomers sarcophytol
    B
    (2) and J (10) were determined by circular dichroism study of their bis-p-dimethylaminobenzoate derivatives. Sarcophytols
    F
    (11) and T (13) were 1
    E
    - and 1
    E
    ,
    3
    Z-isomers of 1. Compound 11 showed characteristic broadening of the ^1H-NMR chemical shifts, due to the restricted conformational interconversion. Using the three cembranoids sarcophytols
    F
    (11, 1
    E
    ,
    3
    E
    ), N (15, 1Z,
    3
    Z) and T (13,1
    E
    ,
    3
    Z), spontaneous autoxidation-cyclization, in CHCl_
    3
    ,was examined, in order to compare the stereochemical course of the reaction with that of 1 (1Z,
    3
    E
    ), which affords trans-fused bicyclo[
    9
    .
    3
    .0]tetradecene systems. The 1
    E
    ,
    3
    Z-isomer 13 gave the same cyclization product 18, as in the reaction of 1, even though it is isomeric at C-1,
    3
    . The 1Z,
    3
    Z isomer 15 gave 19, in consequence of the reversed geometry at C-
    3
    of 15. The 1
    E
    ,
    3
    E
    -isomer (11) gave the bicyclic product
    22
    , having an antipodal fusion as compared with 19. This was confirmed by PCC oxidation of 19 and
    22
    , giving enantiomeric ketones 23 and 24 respectively. The 1(14)-epoxide 26 was shown to be the immediate precursor of
    22
    , and acounted for the inversion of the geometry at C-1 of the cyclization product. Compound 26 is isomeric with the epoxide 17 derived from 1. The epoxide 17 is the postulated precursor in the conversion of 13 to 18. It is noteworthy that the mode of antipodal fusion of the cyclopentane rings, in 18 and
    22
    , was controlled by the chirality of the epoxy rings. The C-14 hydroxyl group participates in the transannular cyclization, but was found not to be the requisite functional group for the reaction. Similar treatment of cembrene C (30), the parent hydrocarbon of 1, also reacted in CHCl_
    3
    giving the bicyclic product 32.
  • 佐藤 敬之輔
    デザイン学研究
    1970年 1970 巻 11 号 45-55
    発行日: 1970/03/30
    公開日: 2017/07/25
    ジャーナル フリー
    [1] The purpose of this reserch is to make clear what type of design can give good effects to win the high readership score. At the begining I had to know what factors decide the readership score, and how to change the quality of the desing into numerical value. I have used the method of multi-dimentional analysis with the help of a computer, and got the estimation formula, with
    7
    kinds of factors, as follows ; A^^〜=
    B
    +C+D+K+aE+bF+cG. A^^〜=estimated values of the readership score. 1)
    B
    , C, D, K : these four factors are given as category values (so we call them); each factor is classified into several categories.
    B
    =week day, classified into
    7
    categories. C=pages of a newspaper, which are characterized by the kinds of articles, news, and the other reading matters, classified into
    22
    categories. D=kinds of goods and trades of the advertizement, classified into 14 catedories. K=constants, given to each 13 surveys. 2)
    E
    ,
    F
    , G : these factors are given as numerical values. G=area of advertizement, measured by the unit of column in the whole page length, being classified into 6 kinds of area (2.
    5
    ,
    3
    .
    5
    ,
    5
    ,
    7
    , 10, 15) and 15 column means a whole page, about 2,000cm^2.
    E
    ,
    F
    are the marks obtained in the design of advertizements.
    E
    =sum of the marks obtained in each design element on the advertizement.
    F
    =the marks obtained on the whole effect of the design.
    3
    ) a,
    b
    , c : coefficients Using the above formula with the category values and the coefficients, we can obtain the naked design effect from the actual value A of readership score: aE+bF=A-(
    B
    +C+D+K+cG). In this research, I used 1,829 data of readership score from 1960 to 1968, being obtained by 13 survevs, spring and autumn twice a year. The sample of size each survey was
    3
    ,541, 2,251, or 200 in the other 11 surveys. Multiple correlation coefficient between the estimated value A^^〜 and the actual value A is 0.951. The table 2^* shows the contribution indexes of each factor by
    3
    kinds of expression-(1) Range : the absolute difference between the maximum and the minimum category values, (2) Standard deviations of the category values, (
    3
    ) Partial correlation coefficient : the relationship between the actual values A and each factors. *see the table 2 in the thesis in Japanese. [2] The important point was in the determination of the values of
    E
    ,
    F
    .
    E
    is the sum of the marks obtained in each design element, classified into four kinds :
    E
    =
    E
    _1+
    E
    _2+
    E
    _
    3
    +
    E
    _
    4
    . design elements [table]
    E
    _1,
    E
    _2,
    E
    _
    3
    ,
    E
    _
    4
    , these values have the
    5
    grades, as 0, 1, 2,
    3
    ,
    4
    and 0 is given to the design that has no attractive effect or no applicable element. And
    E
    obtain the marks of 0 to
    9
    grades as the sum of them. The values of
    F
    have also
    5
    grades, 0 to
    4
    . The principles to determine the values of
    E
    ,
    F
    are as follows : a) The marks obtained of
    E
    ,
    F
    must be the relative values among the each survey, and at the same time, they must have constancy within the same survey-the same elements of design must win the same marks obtained.
    b
    ) They must be determined as to win the highest multiple correlation coefficient, when they are put into the estimation formula. c) They must be reasonable. In order to justify them, we must carry many researches on the actual condition. The frequency of accurence of each grade of
    E
    ,
    F
    values, as the table
    5
    -
    4
    , and
    5
    -
    5
    in the thesis in Japanese.
  • 広田 鋼蔵, 三道 喜一郎, 畑田 元義
    日本化學雜誌
    1960年 81 巻 1 号 29-33
    発行日: 1960/01/10
    公開日: 2011/05/30
    ジャーナル フリー
    エチレングリコールにγ-線照射を行なうと, (a)水素, (
    b
    )メタン, (c)-酸化炭素, (d)エタン, (
    e
    )アセトアルデヒド, (
    f
    )メタノール, (g)エタノール, (h)酢酸, (i)酢酸ヱチル, (j)アセトアルデヒドエチレンアセタール,(k)グリコールモノアセテートおよび(l)水が生成する。これら生成物のG値は,吸収エネルギーとともに,(
    f
    )・(
    e
    )・(h)は不変, (
    b
    )・(d)・(g)・(i)は増加,(a)・(j)・(l)は減少する。また1.
    5
    ×1021ev/ml のエネルギー吸収の際の,それらのG値はつぎのとおりである。G(a)=1.
    9
    , G(
    b
    )=
    9
    .
    7
    ×10-2, G(c)=1.
    5
    ×10-2, G(d)=
    5
    .
    8
    ×10-2, G(
    e
    )=
    4
    .0, G(
    f
    )=1.
    7
    , G(g)=2.
    5
    , G(h)=1.1, G(i)=
    5
    .
    7
    , G(j)=48, G(l)=
    83
    これら生成物のうち, (a)・(
    b
    )・(
    e
    )・(
    f
    )は1次生成物で, 他は2次生成物と推論される。
  • 板倉 智敏, 五藤 精知, 藤原 義昭
    日本獸醫學雜誌(The Japanese Journal of Veterinary Science)
    1973年 35 巻 4 号 289-297_5
    発行日: 1973/08/25
    公開日: 2008/02/13
    ジャーナル フリー
    All the birds examined were also associated with bone dysplasia (art alczaormal develop-ment of bone tissue) in the diaphyseal cortex of the leg bones, especially of bones affectedwith dyschondroplasia. This result is very significant in order to clarifyz tlais disease entity.1970:J14, k -1 }
    7
    )
    7
    , M77 ) i? ht /, ( K )
    7
    (C # L"C, MB @ (
    7
    ) t, If!TB& U "C, Jff Dyschondroplasia (1*t%
    7
    fi!dW) ().g:#
    f
    [!:.fLlt! K?
    3
    o, [1: (
    7
    )M;tC, rff.?U/:cD
    8
    , bb=1lC#li6*H(DX15RI!!D& t, r, ccl)Efff
    7
    flr.#.??t"ut:2.?T<
    3
    .1.?l?
    F
    ?/?t:>"A??1!? >
    E
    >?TMOll?, lI]%G (
    f
    -srRM) -ts;
    5
    ;
    e
    .*BI) *M[t fL 54
    5
    t:.;*.{1*I[l?(), t@Yllll*fJ?H@(
    7
    ) %?Jfipj@ (Dyschondroplasia) p, }, (, 1lTi{-X, WIKIM itt{
    b
    =
    b
    t D"tl%tff{tO 1111IH(-UP!z
    8
    U-ii) >ES- -)%Wi*fd?1J*[lEj: >11"i6ifI".*"H*Tl*&Z") #)/2?-"k l
    E
    B
    - vich ) J&it(Vh
    3
    iO"= >If-ffi"!1lYl*I
    4
    ")Ji/l![40111!>D
    9
    *
    F
    !)<Y14M)?A"!>CL(Il], k
    7
    .Cl
    8
    T"ltTt*flB1? & $
    4
    U [-" C U" L % w, Zl1*.IlBl tI1J, FA(y L-x") LVfAK t: >
    3
    llltPJ
    4
    IiI " } ) t- ! Al j: >kP11liR6, &ffl(C
    5
    NWi Z
    3
    lk(C#L" Ct ?
    f
    !
    4
    M2"C- fL#, -()%
    E
    .IWMd1.XI1*I1EPW(tit ff1lR)
  • Charles-Henri de NOVION, Paul COSTA
    Journal of Nuclear Science and Technology
    1971年 8 巻 10 号 600-601
    発行日: 1971/10/25
    公開日: 2008/12/19
    ジャーナル フリー
  • 鈴木 敏弘
    日本内分泌学会雑誌
    1960年 36 巻 6 号 1075-1091,925
    発行日: 1960/09/20
    公開日: 2012/09/24
    ジャーナル フリー
    Metabolism of body fluids and electrolytes which related not only to aldosterone alone but to other corticosteroids, for example, glucocorticoids had mineral action. And the simultaneous administration of aldosterone and corticosterone consistently resulted in a greater potassium diuresis than did the aldosterone alone.
    Therefore, body fluids and electrolytes are aummation of many corticosteroids which had biological action, and can't know by the determination of simple corticosteroids.
    The author report on the systemic determination of aldosterone, Comp.
    B
    , Comp.
    E
    , Comp.
    F
    , and the newly separated contaminants.
    Method :
    The 24 hours urine specimen is pooled at
    5
    °C and acidified to pH 1.
    5
    with conc. HCL, then standing 24 hours at 15°C.
    Urine was extracted by chloroform and dried. Extract was treated with Florisil column chromatographies and
    4
    consecutered and different paper chromatographies with Chloroform-Formamide System, Bush-C System,
    E2B
    System, and Bush-
    B
    5
    System, to separated corticosteroid partitions.
    Results :
    1. Recoveries of
    5
    mg/100 ml H2O standard samples after
    5
    times continued extraction by 0.1 volume chloroform, for Comp.
    B
    , Comp.
    E
    , and DOC is 95%, and Comp.
    F
    is
    83
    %.
    2. Additional chloroform re-extraction, Comp.
    B
    , Comp.
    E
    , and DOG do not appear in the
    7
    th chloroform extraction, but Comp.
    F
    appear in the amount of 60μg in
    8
    th chloroform and
    9
    th extraction is nothing, thus this determination necessitate at least
    8
    consecutered extractions.
    3
    . Comp.
    B
    , Comp.
    E
    , and Comp.
    F
    appear in 25% methanol in chloroform fraction by the use of the Florisil column chromatography is made, and recovery is about 100% in condition of 60 drops/minutes.
    4
    . Separate Comp. III, Comp. V, Comp. VI, and Comp. VII from aldosterone partition in Neher-Wettstein's method.
    5
    . New contaminants were discover from Comp.
    E
    and Comp.
    F
    partition after Bush-C System paper chromatography, called Comp.
    E2
    -
    E42
    , Comp.
    F2
    ; and, other conatminants named M1-
    M8
    Z1-
    Z8
    , HC1-
    HC3
    , AC1 and AC2 in Chloroform-Formamide and Bush-C System paper chromatography.
    6. Examination of the purities of Comp.
    B
    , Comp.
    E
    , Comp.
    F
    , and aldosterone partitions is made use of the ultra violet absorption spectrum, and necessitates
    4
    (C-
    F
    →Bush-C→
    E2B
    →Bush-
    B5
    ), or
    3
    (C-
    F
    E2B
    →Bush-
    B5
    ), paper chromatographies to satisfactorily separate these compounds.
    7
    . The
    H2SO4
    chromogen spectrum of these partition correspond with the standard compounds.
  • 東坂 諒哉, 城 克己, 林 侑加子, 津村 ゆかり
    日本法科学技術学会誌
    2023年 28 巻 2 号 197-203
    発行日: 2023年
    公開日: 2023/07/31
    [早期公開] 公開日: 2023/04/20
    ジャーナル フリー

     The stability of

    Δ9
    ‒tetrahydrocannabinol acetate (
    Δ9
    ‒THC‒OAc) and
    Δ8
    ‒tetrahydrocannabinol acetate (
    Δ8
    ‒THC‒OAc) in
    e
    -liquid during storage was investigated.
    Δ9
    ‒THC‒OAc liquids in cartridges were stored at 80℃ or 30℃ for 28 days. The relative concentrations of cannabinoids in the liquids were measured by gas chromatography with flame ionization detection.
    Δ9
    ‒THC‒OAc decreased during storage at either temperature.
    Δ9
    ‒THC, a minor impurity, also decreased.
    Δ8
    ‒THC‒OAc liquid in a cartridge was stored at 80℃ for 57 days. The concentration of
    Δ8
    ‒THC‒OAc and
    Δ8
    ‒THC, a minor impurity, did not change during that time. Cannabinol acetate increased in all liquids, and unknown products were also detected by gas chromatography/mass spectrometry (GC/MS). The
    Δ9
    ‒THC‒OAc and
    Δ8
    ‒THC‒OAc liquids were subjected to GC/MS to detect representative diluents, glycerol, propylene glycol, and polyethylene glycol 400, but none of these were detected.

feedback
Top