Abstract
We prepared carotenoid rich pulp by centrifuging Satsuma mandarin juice twice. The freezing and thawing treatment was examined in order to increase carotenoid quantity included in the pulp. After the freezing and thawing treatment was applied to the pulp, a centrifugal separation was carried out. However, the effect which raised the carotenoid quantity of the pulp was low in this treatment because of a lot of soluble solid included in the pulp. Then the freezing and thawing treatment was carried out against the pulp with an addition of 0.1% pectinase. The carotenoid quantity in the sediment could be increased, when the treated pulp was centrifuged. lt is considered that this increase results from the cooperative effect of the enzyme reaction at low temperatures and aggregating the pulp by the freezing and thawing treatment. The freezing and thawing treatment combined with pectinase treatment is practical because it is easy to operate in a large scale manufacture. Also, we can easily manage this process in or out of the Satsuma mandarin juice extraction season.
