Genetic and antigenic characterization of bovine viral diarrhea viruses          isolated from cattle in Hokkaido, Japan

Yuri ABE; Tomokazu TAMURA; Shiho TORII; Shiho WAKAMORI; Makoto NAGAI; Kazuya MITSUHASHI; Junki MINE; Yuri FUJIMOTO; Naofumi NAGASHIMA; Fumi YOSHINO; Yukihiko SUGITA; Takushi NOMURA; Masatoshi OKAMATSU; Hiroshi KIDA; Yoshihiro SAKODA

doi:10.1292/jvms.15-0186

Virology

Genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in Hokkaido, Japan

Yuri ABE, Tomokazu TAMURA, Shiho TORII, Shiho WAKAMORI, Makoto NAGAI, Kazuya MITSUHASHI, Junki MINE, Yuri FUJIMOTO, Naofumi NAGASHIMA, Fumi YOSHINO, Yukihiko SUGITA, Takushi NOMURA, Masatoshi OKAMATSU, Hiroshi KIDA, Yoshihiro SAKODA

Author information

Yuri ABE
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Tomokazu TAMURA
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Shiho TORII
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Shiho WAKAMORI
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Makoto NAGAI
Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183−0057, Japan
Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183−0057, Japan
Kazuya MITSUHASHI
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Junki MINE
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Yuri FUJIMOTO
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Naofumi NAGASHIMA
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Fumi YOSHINO
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Yukihiko SUGITA
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Takushi NOMURA
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Masatoshi OKAMATSU
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Hiroshi KIDA
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001−0020, Japan
Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Hokkaido 001−0020, Japan
Yoshihiro SAKODA
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060−0818, Japan
Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Hokkaido 001−0020, Japan

Keywords: antigenicity, bovine viral diarrhea virus, E2, genetic diversity

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Supplementary material

2016 Volume 78 Issue 1 Pages 61-70

DOI https://doi.org/10.1292/jvms.15-0186

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Abstract

In our previous study, we genetically analyzed bovine viral diarrhea viruses (BVDVs) isolated from 2000 to 2006 in Japan and reported that subgenotype 1b viruses were predominant. In the present study, 766 BVDVs isolated from 2006 to 2014 in Hokkaido, Japan, were genetically analyzed to understand recent epidemics. Phylogenetic analysis based on nucleotide sequences of the 5′-untranslated region of viral genome revealed that 766 isolates were classified as genotype 1 (BVDV-1; 544 isolates) and genotype 2 (BVDV-2; 222). BVDV-1 isolates were further divided into BVDV-1a (93), 1b (371) and 1c (80) subgenotypes, and all BVDV-2 isolates were grouped into BVDV-2a subgenotype (222). Further comparative analysis was performed with BVDV-1a, 1b and 2a viruses isolated from 2001 to 2014. Phylogenetic analysis based on nucleotide sequences of the viral glycoprotein E2 gene, a major target of neutralizing antibodies, revealed that BVDV-1a, 1b and 2a isolates were further classified into several clusters. Cross-neutralization tests showed that BVDV-1b isolates were antigenically different from BVDV-1a isolates, and almost BVDV-1a, 1b and 2a isolates were antigenically similar among each subgenotype and each E2 cluster. Taken together, BVDV-1b viruses are still predominant, and BVDV-2a viruses have increased recently in Hokkaido, Japan. Field isolates of BVDV-1a, 1b and 2a show genetic diversity on the E2 gene with antigenic conservation among each subgenotype during the last 14 years.

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