Further evaluation of an ELISA kit for detection of antibodies to a          nonstructural protein of foot-and-mouth disease virus

Katsuhiko FUKAI; Tatsuya NISHI; Kazuki MORIOKA; Manabu YAMADA; Kazuo YOSHIDA; Rie KITANO; Reiko YAMAZOE; Toru KANNO

doi:10.1292/jvms.15-0219

Virology

Further evaluation of an ELISA kit for detection of antibodies to a nonstructural protein of foot-and-mouth disease virus

Katsuhiko FUKAI, Tatsuya NISHI, Kazuki MORIOKA, Manabu YAMADA, Kazuo YOSHIDA, Rie KITANO, Reiko YAMAZOE, Toru KANNO

Author information

Katsuhiko FUKAI
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
Tatsuya NISHI
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
Kazuki MORIOKA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
Manabu YAMADA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
Kazuo YOSHIDA
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
Rie KITANO
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
Reiko YAMAZOE
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
Toru KANNO
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan

Keywords: antibody, ELISA, foot-and-mouth disease virus, nonstructural protein, vaccine

JOURNAL FREE ACCESS

2016 Volume 78 Issue 3 Pages 365-373

DOI https://doi.org/10.1292/jvms.15-0219

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Abstract

An ELISA kit for detection of antibodies to a nonstructural protein of foot-and-mouth disease (FMDV) was further evaluated using sequentially collected serum samples of experimentally infected animals, because the sensitivity of the kit used in a previous study was significantly low in field animals. The kit fully detected antibodies in infected animals without vaccination; however, the first detections of antibodies by the kit were later than those by the liquid-phase blocking ELISA that is used for serological surveillance in the aftermath of outbreaks in Japan, for detection of antibodies to structural proteins of FMDV. Additionally, although the kit effectively detected antibodies in infected cattle with vaccination, there were several infected pigs with vaccination for which the kit did not detect antibodies during the experimental period. Taken together, the kit may not be suitable for serological surveillance after an FMD outbreak either with or without emergency vaccination in FMD-free countries.

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