材料親和性ペプチドを利用した機能性タンパク質の部位選択的吸着と配向制御

抄録

Proteins including antibody, enzyme and so on have a huge potential to develop a number of new functionalmaterials when they are immobilized on the artificial solid surfaces. Protein immobilization technologieshave been studied in the fields of biological chemistry and/or bioprocess engineering, while understandingadsorption phenomena and/or development of new immobilization strategies are still recognized to beimportant for improvement of density, orientation and biological activity of proteins after their immobilizationespecially in the fields of immunological diagnostics and regenerative medicine. On the other hand, whentarget proteins are immobilized on the surfaces of target solid materials, proteins often suffered from seriousconformational changes, followed by decreasing biological activities significantly. Even when we tried toimmobilize proteins via chemical coupling, similar tendency has been observed. Therefore, new strategies formaintaining density and orientation of functional proteins, and for remaining their activity at higher levelsare requisite. In this review, a new protein immobilization strategy using material-binding peptides which canrecognize the surfaces of solid materials directly is introduced. Furthermore, some examples of immunologicaldiagnostics using recombinant antibody fragments, such as single-chain Fv fused with material-bindingpeptides were demonstrated.