A Simple Media for Growth of Spodoptera litura (Lepidoptera: Noctuidae) Cells and Propagation of Autographa californica Nuclear Polyhedrosis Virus

抄録

Four different commercially produced insect media, including TNM-FH, Grace's MM, and TC-100 were used to culture a cell line, SL7B, derived from Spodoptera litura. It was shown that SL7B cells can grow successfully in all tested insect media except TC-100. The population doubling times were 25.6 h 74.0 h, 56.7 h, respectively in TNM-FH, Grace's, and MM insect media. A simple medium, ISC-03, slightly modified from MITSUHASHI's MTCM-1601, was suitable for culturing SL7B cells. the component of ISC-03 based on diluted sea water, yeast extract, lactalbumin hydrolysate, and glucose. ISC-03 medium is easy to prepare and can be sterilized by autoclaving. The cost is much lower than other commercially available insect cell culture medium. eight percent FBS was added to the medium just before to culture the cells. The population doubling time of SL7B cells in ISC-03 medium was 60.7 h. When cells were grown in ISC-03 medium, the numbers of occlusion bodies formed and recombinant proteins expressed were the same or slightly less than for TNM-FH insect medium. Results revealed that the low cost media, ISC-03, was suitable for culture Spodoptera litura cells.