Effects of Diethyldithiocarbamate and Sodium Thiosulfate on the Detection of Lipoprotein Hydroperoxide
1997 年 32 巻 4 号 p. 595-599
詳細
1997 年 32 巻 4 号 p. 595-599
The effects of insect haemolymph melanization inhibitors, deithyldithiocarbamate (DDC) and sodium thiosulfate, on the detection of lipoprotein hydroperoxide were examined. The hydroperoxide group (36μM) of lipoprotein disappeared completely after 2 h incubation with 1 mM DDC. In the presence of 20 mM sodium thiosulfate, only a portion of the lipoprotein hydroperoxide (LDL-OOH) was consumed in 24 h incubation. The hydroperoxide of lipoprotein became sensitive to sodium thiosulfate in the presence of lipoprotein lipase, a digestive enzyme of lipoprotein. In an incubation of lipoprotein with lipoprotein lipase, sodium deoxycholate and sodium thiosulfate, the hydroperoxide content decreased rapidly. The hydroperoxide group in cumene hydroperoxide was degraded in an incubation with sodium thiosulfate, as was the case for the enzyme-digested LDL-OOH. from the data, an accessibility of the reductant to the hydroperoxide group was assumed to affect the degradation of the hydroperoxide. Based on these results, it was suggested that the lipoprotein hydroperoxide could be detected in the presence of sodium thiosulfate, but not with DDC.
