抄録
The effect of the presence of Mg++ and Zn++ upon the alkaline phosphatase activity of decalcified section was demonstrated in the following 4 groups histochemically.
1) Group 1. (non-treated): A weak enzymatic activity was found in articular and hypertrophic cartilage cells in epiphysis.
2) Group 2. (incubation medium containing Mg++ or Zn++ was used): A moderate enzymatic activity was found in articular and hypertrophic cartilage cells. The surface of primary bone trabeculae showed a strong stainability.
3) Group 3. (pretreated for a short time): The enzymatic activity was moderate to high at the surface of epiphysial plate.
4) Group 4. (pretreated for a long time): A strong enzymatic activity was found in the surface of epiphysial plate and of bone trabeculae. The surface of primary bone trabeculae was the strongest stainability.
The difference of staining intensity between using Mg++ and Zn++ as metallic ion could not be demonstrated. Alkaline phosphatase activity of the decalcified hard tissue was increased by the presense of Mg++ and Zn++ histochemically.
