抄録
Two different techniques, enzyme-cytochemical and immunocytochemical, have been employed to localize alkaline phosphatase in the rat small intestine at light and electron microscopic levels.
In the absorptive cells of the duodenum and jejunum, alkaline phosphatase has been localized by the classical enzyme-cytochemical technique on the surfaces of apical microvilli and lateral membranes; on the other hand the direct immunoperoxidase method revealed some additional sites of the enzyme such as cisternae of rough endoplasmic reticulum and perinuclear spaces. Both enzyme- and immunocytochemistry failed to demonstrate the enzyme in the absorptive cells of the ileum. These immunocytochemical findings suggest that the absorptive cells in the proximal small intestine synthesize and transport the enzyme protein to their cell surfaces.
