抄録
A concurrent microchemical and topochemical study of the tetrazolium reduction by isolated chloroplasts of Vicia faba under illumination was carried out. The microchemical analysis with INT* revealed that the photosystem responsible for the reduction was the PS-II, because the addition of DCMU, DCPA, MCC, CIPC or CAT to the reaction medium at low concentrations markedly suppressed the formazan production. The topochemical analysis with BSPT, combined with the densitometric one, revealed that the electron transfer activity mediated by the PS-II was localized in the thylakoid membrane proper, and extended to the surface of the loculus. This result is in harmony with the spatial localization of the PS-II complex particles on the EF face and the ES of the thylakoid membrane as demonstrated by the freeze fracture technique.
