ENZYME CYTOCHEMICAL STUDY OF RAT CARDIAC MUSCLE II. CA++-ATPASE AND OUABAIN-SENSITIVE, K+-DEPENDENT p-NITROPHENYLPHOSPHATASE
1982 年 15 巻 3 号 p. 338-354
詳細
1982 年 15 巻 3 号 p. 338-354
Myocardial Ca++-activated adenosine triphosphatase (Ca++-ATPase) and ouabain-sensitive, K+-dependent p-nitrophenylphosphatase (K+-NPPase) were demonstrated by the method of Ando et al. (1981) and Mayahara et al. (1980). At pH 9.0, Ca++-ATPase activity was localized on the myofilaments, sarcoplasmic reticulum, gap junction, and the matrix of mitochondria. Sarcolemmal ATPase activity could be detected at neutral pH. K+-NPPase was seen on the cytoplasmic side of the sarcolemma and T-tubule. These findings support the role of these structures for intracellular Ca++-Na+ (probably K+ also related) interaction inducing the heart activity.
