ACID PHOSPHATASE ACTIVITIES IN THE TRANSITIONAL EPITHELIUM OF CANINE URINARY BLADDER

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The acid phosphatase activities in the transitional epithelium of the dog urinary bladder were examined ultracytochemically using a modified technique of Gomori with β-glycerophosphate as the substrate. The reaction products were localized on the plasma membrane in addition to the lysosomes and Golgi complexes. This enzyme activity was sensitive for tartrate, sodium fluoride, and heat treatment and insensitive for alloxan, p-chloromercuribenzoic acid and magnesium chloride. No prominent difference between lysosomal and extra-lysosomal acid phosphatase activities was observed. Since the possible participation of alkaline phosphatase was ruled out by use of the appropriate inhibitors, the enzyme reactions on the plasma membrane may be interpreted as reflecting the lysosomal acid phosphatase.