Abstract
In order to determine DNA content of leukemic cells by means of cytofluorometry, morphological identification of cells on the Wright-Giemsa (W-G) stained smear is required prior to Feulgen nuclear reaction. The complete removal of W-G staining is an essential problem to perform accurate DNA cytofluorometry. This paper reports the most reliable destaining method which preserves the proportionality between fluorescence intensity and DNA content based on a comparative study of several conventional destaining methods.
The destaining method with 5% TCA followed by 100% methanol is considered to be the most practical and reliable method to remove W-G staining on the accurate DNA cytofluorometry of the cells identified by W-G stain.
