All issues
Volume 16, Issue 1
Displaying 1-9 of 9 articles from this issue
- |<
- <
- 1
- >
- >|
-
ATSUSHI KOHNO, KEN-ICHI SEKITA, YOSHIHIRO HAMASHIMA1983 Volume 16 Issue 1 Pages 1-12
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSTo analyze the characteristics of the immune deposits related to anti-DNA antibodies on the glomeruli of MRL/MP-1pr/1pr (MRL/1) mice, an elution study and an immunofluorescent study using antiultravioletirradiated (uv) DNA antisera (rabbit) were carried out. MRL/1 mice developed a high concentration of immunoglobulin G (IgG), anti-DNA antibodies, rheumatoid factors (RF), and circulating immune complexes (CIC) in the sera. In contrast, RF and IC were not significantly detected in the renal eluates in MRL/1 mice, although anti-DNA antibodies revealed high titers, while the titers of anti-dsDNA antibodies in the sera and eluates after DNase digestion did not increase significantly. Furthermore, DNA were not detected as the antigens (Ag) on the glomeruli by the immunofluorescent study using anti-uvDNA antisera which are specific to DNA Ag, though we detected anti-DNA antibodies in the eluates in MRL/1 mice. These results suggest either that other antigen-antibody systems constitute the major IC component in lupus nephritis of MRL/1 mice or that DNA Ag fixed to the glomeruli was digested with endogeneous DNase into oligonucleotide which is resistant to treatment with DNase, and dose not produce comformational changes after uv-irradiation.View full abstractDownload PDF (3112K) -
CHIHIRO SHIMAZAKI, SHINOBU NAKANISHI, HARUE HARUYAMA, TAKUJI ISEMURA, ...1983 Volume 16 Issue 1 Pages 13-21
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSIn order to determine DNA content of leukemic cells by means of cytofluorometry, morphological identification of cells on the Wright-Giemsa (W-G) stained smear is required prior to Feulgen nuclear reaction. The complete removal of W-G staining is an essential problem to perform accurate DNA cytofluorometry. This paper reports the most reliable destaining method which preserves the proportionality between fluorescence intensity and DNA content based on a comparative study of several conventional destaining methods.
The destaining method with 5% TCA followed by 100% methanol is considered to be the most practical and reliable method to remove W-G staining on the accurate DNA cytofluorometry of the cells identified by W-G stain.View full abstractDownload PDF (1866K) -
YOSHITERU KATSUKURA, TAKESHI TSUCHIYA, NOBUYUKI ABE, NOBUYUKI WATABE1983 Volume 16 Issue 1 Pages 22-30
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe localization of fibronectin in the aortic intima from 2, 4 and 8 months old SHR were observed with immunoperoxidase technique. Acid polysaccharide stainings were consistently performed on the serial sections of the same specimen.
In 2 months old rats, aortic intima showed normal structure arrangement. In such cases, fibronectin localized linearly in the basement membrane underlying epithelium. The intima from rats of over 4 months of age tended to thicken with fibrin-like and fibrous substances, and injury to epithelium was often observed. In these cases, reaction products appeared to be deposited from plasma fibronectin and by secretion of migrated cells from the media. Acid polysaccharides positive area was similar to the portions of fibronectin deposits. Some discussion was held on the pathogenesis of the primary stage of arteriosclerosis correlated with fibronectin mobilization.View full abstractDownload PDF (5698K) -
NORIHISA GOSHI, OSAMU FUKUSHIMA1983 Volume 16 Issue 1 Pages 31-40
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe lysosomal enzyme of osteoclasts which hydrolyze ATP or ADP at the neutral pH range (pH 7.0-7.4) was markedly inhibited by NaF. On the other hand, L-tetramisole or L-cysteine, known to be the inhibitors for the alkaline phosphatase, stabilized or rather increased the enzyme activity. The degree of the activity was moderate to intense when either of them was used solely or with NaF. The thiamine pyrophosphatase activity was also influenced by the above mentioned agents equally as the cases of ATP-or ADP-hydrolysis. The activity of ATP-hydrolysis in lysosomes at pH 8.5 was generally faint, and the inhibitory effect of the same agents seemed to be very weak or noneffective. These histochemical findings led us to conclude that the enzyme dealt with was possibly the neutral pyrophosphatase indicated by the biochemical procedures.View full abstractDownload PDF (6921K) -
YASURO ATOJI1983 Volume 16 Issue 1 Pages 41-50
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe uptake of 3H-GABA by the Purkinje cells in the rat embryo was examined by light and electron microscopic autoradiography. The developing Purkinje cells became first labelled with 3H-GABA at E-18 when they began to form the primordium of the Purkinje cell layer, and continued to be labelled until E-22 (at term). From E-18 to E-20 most of the Purkinje cells were heavily labelled, but at E-21 and E-22 the Purkinje cells situated at the top of the cerebellar gyri were heavily labelled while those situated at the bottom of the cerebellar sulci were not labelled. Silver grains on the labelled Purkinje cells were distributed on both the nucleus and the cytoplasm. At E-22 a few unlabelled axon terminals on the labelled Purkinje cell soma and also a few labelled glial processes in the Purkinje cell layer were first detected. Throughout the stages examined no silver grains were found on the external granular layer, on the molecular layer and on the intercellular spaces of the Purkinje cell layer as well as of the mantle layer. These findings indicate that the developing Purkinje cells in rat embryo possess an ability of 3H-GABA uptake before synapses and/or glial wrapping around their soma are formed.View full abstractDownload PDF (7336K) -
TOSHIO HARIGAYA, KAORU KOHMOTO, KAZUMASA HOSHINO1983 Volume 16 Issue 1 Pages 51-58
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSProlactin producing cells in the anterior pituitary glands of normal adult male and female mice were observed immunohistochemically using anti-mouse prolactin rabbit serum. In this study, three types of prolactin cells can be distinguished. The first type contains small spherical secretory granules about 100 nm in diameter, the second type has medium sized spherical secretory granules ranging in diameter from 150 to 200 nm, and the third type has secretory granules, about 300 nm in maximal diameter, in variable shapes and sizes. The shapes of prolactin cells vary from oval, polygonal, stellate to cup-shaped. The Type I and Type III prolactin cells are usually oval or polygonal, but the Type II prolactin cells are mostly irregular. In the Type I and Type II prolactin cells, the cytoplasmic organellae are poorly developed. However, the Type III prolactin cells have well developed endoplasmic reticula and enlarged Golgi apparatus. The Type III prolactin cells are most likely the same as the anterior hypophysial cells described as mammotrophs or lactotrophs by earlier investigators using morphological criteria. However, the Type I and Type II cells identified by the present immunohistochemical study using anti-mouse prolactin rabbit serum have never previously been recognized as prolactin cells by non-immunohistochemical methods. Sex differences are observed in the proportion and the frequency of the occurrence of these three types of prolactin cells in the anterior pituitary gland of the mouse: among three types of prolactin cells, the most predominant type being Type III in female mice, whereas Type II in male mice.View full abstractDownload PDF (6213K) -
FRANCESCO AMENTA, ALESSANDRO CANTAGALLI, CARLO CAVALLOTTI, CARLO DE LU ...1983 Volume 16 Issue 1 Pages 59-65
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe innervation pattern of three peripheral muscular arteries superficial epigastric, saphenous and tail artery was analyzed in the rat.
Some rats were sympathectomized by use of neurotoxin 6-hydroxy-dopamine (6-OHDA) while other normal animals were used as controls. Pieces from the arteries were incubated in a quinacrine (5×10-7 M) containing solution in order to visualize nerve fibers with a selective affinity for the drug (likely peptidergic in nature) or processed for the histochemical detection of peripheral stores of catecholamines or of the acetylcholinesterase activity.
No nerve fibers with selective affinity for quinacrine were found, while arteries studied appeared to be supplied with adrenergic and acetylcholinesterase-containing nerve fibers.
The 6-OHDA treatment caused the almost complete disappearance of adrenergic and acetylcholinesterase-containing nerve fibers within the three arteries.
The findings suggest that rat superficial epigastric, saphenous and tail arteries are supplied only with a sympathetic innervation. The possible physiological significance of the presence of an acetylcholinesterase activity in sympathetic nerves is discussed.View full abstractDownload PDF (2564K) -
JUAN CARLOS STOCKERT1983 Volume 16 Issue 1 Pages 66-69
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (414K) -
YUJI MIZUKAMI, FUJITSUGU MATSUBARA1983 Volume 16 Issue 1 Pages 70-73
Published: 1983
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (1245K)
- |<
- <
- 1
- >
- >|