DOUBLE STAINING OF INDIRECT IMMUNOPEROXIDASE METHOD FOR HCG AND AFP IN THE HUMAN TESTICULAR TUMOR
1983 年 16 巻 2 号 p. 148-154
詳細
1983 年 16 巻 2 号 p. 148-154
The present investigation is aimed at showing the localization of human chorionic gonadotropin (hCG) and alpha-fetoprotein (AFP) in the same section of human testicular carcinoma using successive double staining by the indirect immunoperoxidase method. Specimens from 3 cases of human testicular tumors with elevated serum hCG and AFP values were fixed in 95% ethanol or 10% buffered formalin. Both anti-hCG-β and anti-AFP rabbit sera were absorbed with several materials in order to avoid cross reaction in the double staining. Indirect immunostaining was performed using anti-hCG-β or anti-AFP serum. HRP activity was demonstrated by reacting with either 3, 3′-diaminobenzidine, 4-Cl-1 naphthol and 4-Cl-1 naphthol-pyronin. HCG- and AFP-localizing cells could be completley differentiated from each other. HCG was localized in the cytoplasm of the syncytiotrophoblastic cells, syncytiotrophoblastic giant cells and embryonal carcinoma cells. AFP was observed at endodermal sinus tumor elements, embryonal carcinoma cells and teratoma elements.
