PROBLEMS OF LIGHT MICROSCOPICAL ADENYLATE CYCLASE DEMONSTRATION IN RAT CEREBELLUM WITH ADENYLYL IMIDODIPHOSPHATE
1984 年 17 巻 5 号 p. 603-611
詳細
1984 年 17 巻 5 号 p. 603-611
Adenylate cyclase in rat cerebellum was investigated on the light microscopic level with adenylyl imidodiphosphate (AMP-PNP) as substrate. The results show that the reaction is overlapped by other enzymes with high activity and inhibited by NaF and dithiothreitole. It does not seem possible to demonstrate adenylate cyclase light microscopically in rat cerebellum because adenylate cyclase activators inhibit the conversion of AMP-PNP. The biochemical results of Johnson and Welden (7) could be confirmed histochemically.
