Abstract
Tissue and organ distribution of radioactive carbon from 14C-tyrosine in the mouse was studied by whole-body autoradiography and biochemical analysis. The mice injected intravenously with L-[U-14C]tyrosine were sacrificed at various intervals. Examination of autoradiographs disclosed that the injected 14C-tyrosine was rapidly taken up from the blood by the tissues. The radioactivity in the pancreas was the highest and predominant throughout the intervals after injection in this investigation.
The comparative values among radioactivity in the organs estimated by a liquid scintillation counter were approximately consistent with those obtained from the whole-body autoradiographs. Radioactivity in the acid-insoluble fractions was increased with time in all organs examined. High-performance liquid chromatography of the acid-soluble fractions disclosed that the amounts of the radioactive tyrosine and its metabolites and/or their molar ratios were different among the organs.
