Abstract
As part of a search for a new staining agent with higher selectivity for particular metals, we examined the staining properties of thiazolylazo-benzoic acid derivatives for histochemical staining of metals in rat liver tissue. Thiazolylazodimethylaminobenzoic acid (TAMB) stained only two metals, palladium(Pd) and copper(Cu), among a variety of metals. The results obtained revealed that the TAMB method was superior to the conventional p-dimethylaminobenzylidenerhodanine method with regard to selectivity for various metals when staining for Pd in histochemical practice. The differentiation of Pd from Cu was achieved by using masking agents, pyrophosphate or tripolyphosphate.
