抄録
In situ hybridization histochemistry with synthetic oligodeoxynucleotide probes was applied to investigate the localization of tissue mRNAs. Specific probes for mRNAs to vasopressin- and oxytocin-preprohormone were designed by using the computer-based homology search program. The probes were labeled with 3H- deoxycytidine triphosphate (dCTP) or 35S-deoxyadenosine triphosphate (dATP) by terminal deoxynucleotidyl transferase. The specificity of the tailed probe was again confirmed by the homology search. Issues for the properties of isotopes and data analysis of the hybridizatioin signals in the autoradiography were discussed from the methodological point of view.
