抄録
Fracture-flip to provide extended views of the membrane surfaces at close to macromolecular resolution has been developed by Pinto da Silva et al. (1988). This method is based on the carbon stabilization of the hydrophobic face of split membrane halves. After thawing, the carbon casts with their membrane halves are flipped, and then the actual membrane surfaces is imaged by platinum shadowing. Fracture-flip can be combined with cytochemical labelling performed before or after freeze-fracturing. We show here the practical procedures for fracture-flip and its application to various cells and cell organelles.
