抄録
The fine ultrastructural localization of acid phosphatase (AcPase) activity in mouse spleen ages; 1 day, 1 and 2 weeks, and 1, 2 and 10 months was carried out by cytochemistry, using cerium as the trapping agent. The AcPase enzyme activity was observed in the red pulp. The enzyme reaction product precipitates appeared dense and homogeneous, and were localized in the lysosomes of macrophages, phagocytic reticular cells and littoral cells. The presence of cerium in the reaction product precipitate was confirmed by X-ray microanalysis. The spectral line was present at La=4.84KeV and did not interfere with osmium emission line at Ma=1.914KeV. The results demonstrate variation in the degree of AcPase activity in different ages of the animal studied. The enzyme activity increased progressively from day one, recorded a peak on the first week and thereon, decreased gradually until the 10th month.
