抄録
We reported previously that fodrin is concentrated in the posterior portion of the human neutrophil stimulated with the chemotactic peptide (Fujimoto and Ogawa, J. Cell Sci., 96: 477-486, 1990). In the present study, the mechanically unroofed specimen of glass-adherent neutrophils was examined to define finer details of fodrin distribution. By immunofluorescence microscopy, F-actin and fodrin were positively labeled; after stimulation with the chemotactic peptide, fodrin was found more densely in the posterior half of the cell as in the whole cell observation. In ultrathin sections of the unroofed and immunogold-labeled specimen, two dimensional distribution of fodrin could be observed. Some gold particles were observed with filaments of about 5 nm in diameter, but others were on amorphous materials. In the stimulated neutrophil, labeling for fodrin was mainly found between straight filaments of about 10 nm in diameter which were densely distributed in the posterior portion of the cell. The results indicate that the redistribution of fodrin in the stimulated neutrophil is concurrent with other cytoskeletal changes.
