Abstract
A single intra peritoneal injection of colchicine to mice causes both functional and morphological changes on Golgi apparatus in pancreatic exocine cells. Structural changes included shrinkage of Golgi area in the secretory cell, whirling of the Golgi cisternae, decrease of the Golgi cisternae and increase of small vesicles around the remnants of Golgi cisternae. Cytochemical distribution of Golgi marker enzyme, nicotinamide adenine dinucleotide phosphatase (NADPase) which was found in the middle cisternae of Golgi stack in control, decreased the activity and expanded to the entire Golgi stack and in most small vesicles. Also by zinc-iodide-osmium (ZIO) stain which preferably stained cis-side of Golgi stack in control, the entire Golgi stacks were stained as well as these small vesicles. From these observation, by colchicine treatment, the NADPase and the osmium-reducing substance in the cis Golgi cisternae, first appeared to expand its distribution to all the Golgi components. Then, Golgi cisternae appeared to reduce the size either by vesiculation of the cisternae or by trimming the cisternae as cisternal whirling into autophagosomes.
