Abstract
Distribution of cytokeratins No. 1-19 detected by MAB f 12-19, No. 1, 2, 9, 10, 11 detected by MABEE 21-06 and desmosome peptides 33-52 kDa of skin granular layer detected by MABs G 36-19 in Bowen's disease was studied by the immunoperoxidase and PAP methods. Monoclonal f 12-19 antibody uniformlystained all layers of normal and lesional epidermis in Bowen's disease.
MAB EE 21-06 in normal epidermis showed a reaction only in suprabasal cells, but in Bowen's disease the intensity of staining was considerably decreased. In normal epidermis, MAB G 36-19 stained granularlayer, while in Bowen's disease the reaction was reduced at the site of active proliferation. Thus the newl ysynthesized monoclonal antibodies allowed us to detect the disturbances in expression of keratinocyte differentiation markers in Bowen's disease.
