抄録
For quantitative determination of Feulgen-DNA in mouse lymphocytes three hydrolysis methods were examined.
With 5N HCl hydrolysis at 25°C, there was found a long plateau of optimum hydrolysis over 30 minutes or more, while the period of optimum hydrolysis in the conventional hydrolysis at 60°C in 1N HCl was a few minutes, and that in 60°C 5N HCl was a few seconds.
Proportionality of DNA content between spermatids and lymphocytes was well preserved over the long hydrolysis time in 25°C 5N HCl hydrolysis.
Therefore, it was concluded that 25°C, 5N HCl hydrolysis was preferable to the conventional Feulgen hydrolysis performed at 60°C with 1N HCl.
