ACTA HISTOCHEMICA ET CYTOCHEMICA
Online ISSN : 1347-5800
Print ISSN : 0044-5991
ISSN-L : 0044-5991
TWO-WAVE-LENGTH-SCANNING METHOD IN FEULGEN CYTOPHOTOMETRY
SETSUYA FUJITAMASARU FUKUDATADAHISA KITAMURASATORU YOSHIDA
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ジャーナル フリー

1972 年 5 巻 3 号 p. 146-152

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抄録
In Feulgen stained specimens, non-specific light loss is always present, which can be detected and measured quantitatively by scanning with a light spot at 450nm. Subtraction of the total “absorbance” at 450nm from that of 550nm virtually eliminates the effects of non-specific light loss and gives more accurate value of DNA content of a Feulgen stained cell. This is called two-wave-length-scanning method. An automatic instrument to perform two-wave-length-scanning measurements is described. By this method measurements were carried out on DNA contents of Purkinje cells and inner granule neurons in cerebellum of man and rat at various stages of postnatal life, and the rule of strict constancy of DNA content is confirmed. Based on discussions on various sources of the non-specific light loss, the use of this method is recommended when high accuracy is required in measurements of Feulgen cytophotometry.
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© the Japan Society of Histochemistry and Cytochemistry
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