抄録
Alkaline phosphatase of bovine kidney origin was purified in the homogenous purity and crystallized from the commercial source. Using the purified enzyme, the localization of alkaline phosphatase was examined by immunohistochemical technique and compared with that demonstrated by histochemical method. Only the cells composing the proximal convoluted tubule contained alkaline phosphatase.
On the electron microscopic level, alkaline phosphatase was concentrated in the brush border. The findings were consistent with the distribution pattern of the proper enzyme visualized by the histochemical method.
