A COMPARATIVE CYTOSPECTRAL STUDY ON VERTEBRATE LEUCOCYTES STAINED BY THE WRIGHT METHOD

抄録

A new method was developed for cytospectral characterization of leucocytes after staining with Wright solution. The spectral types of various vertebrate leucocytes were compared by this method. To characterize vertebrate leucocytes their absorption maximum (A max) and relative absorption (RA) spectra (A/A max, %) were recorded. From the values of A max and the absorptions at 600nm (RA 600) and 460nm (RA 460), the RA spectra were divided into 7 types (I, II-a, II-b, III-A, III-a, III-b, IV). The leucocyte types of individual species could be classified into certain types, but these types differed in different species. However, even in different species, most thrombocytes were classified as type III-A, mammalian eosinophils as type III-b and those of lower vertebrates as type IV. In general the spectral types changed progressively from higher to lower animal species. The blood of lower vertebrates seemed to contain immature cells judging from the RA spectra of these cells. From our data this spectral classification seems promising for comparison of vertebrate leucocytes and should be useful in their cytoanalysis.