To improve indoor air quality, it’s necessary to accurately control the body odor emitted by humans living under various environmental conditions and lifestyles. The purpose of this study was to obtain a better understanding of the effects of changes in physical quantities due to insufficient sleep on the emission of human bioeffluents causing body odor and the perceived air quality. The experiment involved two conditions of sleep time. Different biogenic substance concentrations and sensory evaluations of the odor in the chamber were obtained when the occupants' sleep times was different, even for the same CO2 concentration in the room.