In this study, we investigated the influence of material segregations on the behaviors of high-strength concrete. The test results indicate that the mechanical properties of structural concrete more easily influenced by the material segregation. Material segregation may result in inadequate other performances of the structure using high-strength concrete, besides the degradation of mechanical properties, due to the significant differences in temperature rise and shrinkage. 90%, which is a ratio of actual coarse aggregate area to be the planed one, may be considered as an allowable limit of material segregation. Also, there is no great difference in the performances of structural concrete if the material segregation index of upper layer is not more than 20%.