Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Notes
Ultrafast Diagnosis of the Genetic-related Disorders Using the Combined Technologies of Multiplex PCR and Multichannel Microchip Electrophoresis
Mohammad JABASINIAshraf A. EWISFeng XUGuichen PINGMaged FOUADToshikatsu SHINKAYutaka NAKAHORIMitsuru ISHIKAWAYoshinobu BABA
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2005 Volume 21 Issue 12 Pages 1537-1539

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Abstract

For the diagnosis of unexplained male infertility a multiplex PCR for 6 markers, which are well-known as candidate genes for studying male infertility and located on the human Y-chromosome, has been designed. The multiplex PCR products have been separated on a 12 channel microchip electrophoresis system, which can analyze different samples simultaneously. By combining the technologies of multiplex PCR with multichannel microchip electrophoresis, the number of the DNA markers that can be screened simultaneously is increased to be 72 marker (12 × 6) in a single run while the electrophoresis analysis time is reduced to be only 180 s.

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© 2005 by The Japan Society for Analytical Chemistry
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