Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Notes
The Effect of Local Structural Disruption on the Yield of Photochemical Ligation between Anthracene-Oligonucleotide Conjugates
Pelin ARSLANToshihiro IHARAMotoko MUKAEAkinori JYO
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2008 年 24 巻 1 号 p. 173-176

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The techniques of chemical ligation have attracted great attention as an alternative to enzymatic joining of DNA ends. Here we introduce the photoligation of anthracene-modified ODN conjugates through anthracene cyclodimer formation. The effect of the positions and the kinds of single base mismatch on the template was evaluated using eight templates with one-base displacements. We found out that the yield of the ligation was affected by mispairing in a position-dependent manner. Such results would be attributed to the disruption of the local structure at the ligation site.
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© 2008 by The Japan Society for Analytical Chemistry
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