抄録
An amperometric horseradish peroxidase (HRP) inhibition biosensor has been substantially constructed by the help of N,N-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS). The preparation steps and the biosensor response to phenylhydrazine were monitored by electrochemical impedance spectroscopy (EIS), cyclic voltammetry, and chronoamperometry. The proposed biosensor could be applied to determine phenylhydrazine in a 0.10 M phosphate buffer solution containing 1.2 mM hydroquinone and 0.50 mM H2O2 by phenylhydrazine, inhibiting the catalytic activity of the HRP enzyme in the reduction of H2O2. The system was optimized to realize a reliable determination of phenylhydrazine in the range of 2.5 × 10-7 to 1.1 × 10-6 M with a detection limit of 8.2 × 10-8 M and a correlation coefficient of 0.999. The modified electrode displayed good reproducibility, sensitivity and stability for the determination of phenylhydrazine.
