Abstract
A novel, high-performance wide-bore electrophoresis (WE) system with inner-cooling has been developed. By introducing the mode of a shell and tube heat exchanger into this system to remove Joule heat generated during electrophoresis, it is feasible to extend electrophoresis from the conventional capillary (i.d. <100 µm) to a wide-bore tube (i.d. >1000 µm). The wide tube allows the loading of over 1.0 µL of the sample with an LOD of 3.0 × 10-4 mg/mL (signal-to-noise ratio, 3:1). Satisfactory separations of model compounds have been achieved on the WE system.
