Simple Pretreatment and HILIC Separation for LC-ESI-MS/MS Determination of Adenosine in Human Plasma

Hiroya MURAKAMI; Erina OTANI; Tomoko IWATA; Yukihiro ESAKA; Takuma AOYAMA; Masanori KAWASAKI; Toshiki TANAKA; Shinya MINATOGUCHI; Bunji UNO

doi:10.2116/analsci.31.1189

Notes

Simple Pretreatment and HILIC Separation for LC-ESI-MS/MS Determination of Adenosine in Human Plasma

Hiroya MURAKAMI, Erina OTANI, Tomoko IWATA, Yukihiro ESAKA, Takuma AOYAMA, Masanori KAWASAKI, Toshiki TANAKA, Shinya MINATOGUCHI, Bunji UNO

著者情報

キーワード: Adenosine, human plasma, LC-ESI-MS/MS, ion suppression, HILIC separation, ammonium acetate

ジャーナルフリー

2015 年 31 巻 11 号 p. 1189-1192

DOI https://doi.org/10.2116/analsci.31.1189

詳細

抄録

A simple pretreatment method and separation mode for the LC-ESI-MS/MS determination of adenosine in human plasma have been developed. Deproteinization by acetonitrile and ultrafiltration followed by chromatographic separation with a hydrophilic interaction chromatographic (HILIC) column give a highly sensitive MS/MS response without ionic suppression caused by the matrix compounds in human plasma. In addition, the presence of ammonium acetate in the mobile phase contributes to high sensitivity in MS/MS detection, facilitating the ionization of adenosine. This method seems to be amenable to the treatment of many samples in clinical practice.

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