Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
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Direct Lipido-Metabolomics of Single Floating Cells for Analysis of Circulating Tumor Cells by Live Single-cell Mass Spectrometry
Eiso HIYAMAAhmed ALISara AMERTakanori HARADAKazumi SHIMAMOTORie FURUSHIMAYasmine ABOULEILASamy EMARATsutomu MASUJIMA
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2015 Volume 31 Issue 12 Pages 1215-1217

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Abstract

Direct trapping of a single floating cell, i.e. a white blood cell from a drop of blood, within a nanospray tip was followed by super-sonication after the addition of ionization solvent. Molecular detection of an increased number of peaks with a higher intensity and a wider m/z range, which extends from metabolites to lipids, was acquired than of that without sonication. This method was applied to a few separated circulating tumor cells (CTC) from a neuroblastoma patient’s blood to obtain their lipido-metabolomic molecular profile at the single cell level. In addition to vital molecules such as amino acids, catechol amine metabolites, which are specific to neuroblastoma, and drugs included in the patient’s course of therapy were detected. This established “direct single-cell lipido-metabolomic method” seems to be useful for direct and wide range molecular detection not only for many live single-cells, but also for rare cells, such as CTCs, for future molecular diagnosis.

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© 2015 by The Japan Society for Analytical Chemistry
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