Simulation Analysis of Positional Relationship between Embryoid Bodies and Sensors on an LSI-based Amperometric Device for Electrochemical Imaging of Alkaline Phosphatase Activity

Yusuke KANNO; Kosuke INO; Kumi Y. INOUE; Atsushi SUDA; Ryota KUNIKATA; Masahki MATSUDAIRA; Hitoshi SHIKU; Tomokazu MATSUE

doi:10.2116/analsci.31.715

Notes

Simulation Analysis of Positional Relationship between Embryoid Bodies and Sensors on an LSI-based Amperometric Device for Electrochemical Imaging of Alkaline Phosphatase Activity

Yusuke KANNO, Kosuke INO, Kumi Y. INOUE, Atsushi SUDA, Ryota KUNIKATA, Masahki MATSUDAIRA, Hitoshi SHIKU, Tomokazu MATSUE

著者情報

キーワード: LSI-based amperometric chip device, simulation analysis, cell analysis, electrochemical imaging

ジャーナルフリー
電子付録

2015 年 31 巻 7 号 p. 715-719

DOI https://doi.org/10.2116/analsci.31.715

詳細

抄録

In the present study, we monitored the alkaline phosphatase (ALP) activity of embryoid bodies (EBs) of mouse embryonic stem (ES) cells using a large-scale integration (LSI)-based amperometric device with 400 sensors and a pitch of 250 μm. In addition, a simulation analysis was performed to reveal the positional relationship between the EBs and the sensor electrodes toward more precise measurements. The study shows that simulation analysis can be applied for precise electrochemical imaging of three-dimensionally cultured cells by normalization of the current signals.

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