Effective Separation and Simultaneous Determination of Corynoxeine and Its Metabolites in Rats by High-Performance Liquid Chromatography with Tandem Mass Spectrometry and Application to Pharmacokinetics and In Vivo Distribution in Main Organs

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An effective separation and simultaneous determination of corynoxeine and its metabolites using high-performance liquid chromatography with tandem mass spectrometry was developed and validated. The method was applied to pharmacokinetics and in vivo distribution investigations in rats after oral (0.105 mmol kg⁻¹) and intravenous (0.0105 mmol kg⁻¹) doses of corynoxeine. Its brain uptake index was of 3.08 × 10⁻¹¹ mol g⁻¹ at 3 h and 3.75 × 10⁻¹¹ mol g⁻¹ at 74 min after oral and intravenous doses, respectively.