抄録
An extractive spectrophotometric method for the determination of micro amounts of ascorbic acid has been developed by exploiting its reducing nature. Iron(III) is converted to iron(II) by the addition of ascorbic acid, which forms an extractable yellow-colored complex with picolinic acid and pyridine. The absorbance is measured at 400nm. Beer′s law is obeyed in the concentration range 0.4-5.6μg ml-1. This method has been satisfactorily applied to the analysis of a variety of samples, including pharmaceuticals, food products and biological fluids.
