抄録
A study on the separation and detection of organic and inorganic phosphorus compounds using a capillary electrophoresis (CE) system has been performed. The separation of 18 phosphorus compounds is characterized by good resolution and analysis times less than 20min using EDTA (ethylenediaminetetraacetic acid) electrolyte. The cumulative data demonstrate that it is possible to undertake simultaneously separation of both UV-absorbing and UV-transparent components with EDTA electrolyte. UV-absorbing components were measured at 210nm and UV transparent components indirectly determined at 210nm. The influence of electrolyte (EDTA) concentration, operating buffer pH, separation voltage, temperature, and electroosmotic flow modifier (OFM anion-BT) concentration is described. The optimum CE system has operating parameters of 5mM EDTA, pH 10.3, 20kV of separation voltage, 25°C, 0.15mM OFM anion-BT, 70cm×75μm i.d. fused silica capillary, and UV photometry detection at 210nm.
