Determination of Pheophorbide a and Methyipheophorbide a by Fluorescence High Performance Liquid Chromatography: Availability of Narrow Baseline Method

抄録

A narrow baseline method for the simultaneous determination of pheophorbide a and methylpheophorbide a in core sediment samples has been developed by high performance liquid chromatography. A linear relationship between the concentration of methylpheophorbide a and the peak height on the narrow baseline was obtained. The relative standard deviation of measurement was found to be 2.4% (n=6) for samples containing 8.1×10^-8mol dm^-3 ofmethylpheophorbide a.