Hue Detector for Column Chromatography
1987 年 3 巻 6 号 p. 527-530
詳細
1987 年 3 巻 6 号 p. 527-530
A column chromatograph was made, which has an amorphous integrated diode photo-electric transducer (Sanyo, full color sensor AM 3302) as photodetector. Dyes extracted from spinach leaves were analyzed. AM 3302 has three color (red, green and blue) filters in front of the face of the photo-sensor, and transduces photo-intensities to the voltages. Three voltage outputs are taken into the microprocessor by the rapid sampling method. Three outputs, which correspond to the light intensity at three different wavelengths, form the three point spectrum. AM 3302 can be used as a simple detector of the hue. Extracted dyes from spinach leaves were separated on the silica gel column. The green and two yellow fractions were monitored by the hue detector. The green fraction was fractionated and its fast atom bombardment mass (FAB-MS) spectrum was measured and identified to be chlorophyll a by quasi molecular ion m/z 893 [M+H]+ value.
