Laser Photoionization Detector for Reversed-Phase High Performance Liquid Chromatography

抄録

The laser two-photon ionization technique was applied to reversed-phase high performance liquid chromatography by using a free-falling jet detector (20nl). Methanol, acetonitrile and a mixture of acetonitrile-water (90:10) were used in the mobile phase. The dependences of the detection limit on such experimental parameters as the electrode spacing and applied voltage were measured. The best detection limit was obtained at 0.5mm spacing and at 60V for acetonitrile. Although these solvents showed similar leakage currents, acetonitrile gave the best detection limit. The detection limit was 60pg for pyrene, comparable with or better than that obtained by a UV absorption detector.