Peroxyoxalate Chemiluminescence Assay of Hydrogen Peroxide and Glucose Using 2, 4, 6, 8-Tetrathiomorpholinopyrimido[5, 4-d]-pyrimidine as a Fluorescent Component

Kenichiro NAKASHIMA; Kouichi MAKI; Shinki KAWAGUCHI; Shuzo AKIYAMA; Yukie TSUKAMOTO; Kazuhiro IMAI

doi:10.2116/analsci.7.709

Kenichiro NAKASHIMA, Kouichi MAKI, Shinki KAWAGUCHI, Shuzo AKIYAMA, Yukie TSUKAMOTO, Kazuhiro IMAI

著者情報

キーワード: Peroxyoxalate chemiluminescence, 2,4,6,8-tetrathiomorpholinopyrimido[5,4-d]pyrimidine, hydrogen, peroxide, glucose, glucose oxidase, mutarotase

ジャーナルフリー

1991 年 7 巻 5 号 p. 709-713

DOI https://doi.org/10.2116/analsci.7.709

詳細

Published: 1991/10/10 Received: 1991/06/08 Available on J-STAGE: 2006/06/30 Accepted: 1991/07/09 Advance online publication: - Revised: -
訂正情報
訂正日: 2006/06/30 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : Peroxyoxalate chemiluminescence (CL) assay of hydrogen peroxide (H_{2_{O₂) or glucose was developed by using 2, 4, 6, 8-tetrathiomorpholinopyrimido[5, 4-d]pyrimidine as a fluorescent component and bis(2, 4, 6-trichlorophenyl)oxalate (TCPO) as an oxalate. Linear relationships between CL intensity and final concentration of H₂O₂ from 10^-8 to 10^-4M were obtained. The detection limit at the ratio of CL intensities for sample and blank (S/B) of 3 was 10nM. The precision for five replicate measurements at 10^-5 and 10^-6M of H₂O₂ were 17.6 and 15.7% of relative standard deviations, respectively. α-D-Glucose was transformed to β-D-glucose with mutarotase and converted to H₂O₂ and D-gluconic acid with glucose oxidase, which was detected by using peroxyoxalate CL reaction. A linear calibration graph was obtained up to 1.5×10^-4M of glucose solution. The method was applied to the assay of glucose in human serum. The recovery was 98.2% (n=4). The method correlated well with the conventional colorimetric method (r=0.968).
Right : Peroxyoxalate chemiluminescence (CL) assay of hydrogen peroxide (H₂O₂) or glucose was developed by using 2, 4, 6, 8-tetrathiomorpholinopyrimido[5, 4-d]pyrimidine as a fluorescent component and bis(2, 4, 6-trichlorophenyl)oxalate (TCPO) as an oxalate. Linear relationships between CL intensity and final concentration of H₂O₂ from 10^-8 to 10^-4M were obtained. The detection limit at the ratio of CL intensities for sample and blank (S/B) of 3 was 10nM. The precision for five replicate measurements at 10^-5 and 10^-6M of H₂O₂ were 17.6 and 15.7% of relative standard deviations, respectively. α-D-Glucose was transformed to β-D-glucose with mutarotase and converted to H₂O₂ and D-gluconic acid with glucose oxidase, which was detected by using peroxyoxalate CL reaction. A linear calibration graph was obtained up to 1.5×10^-4M of glucose solution. The method was applied to the assay of glucose in human serum. The recovery was 98.2% (n=4). The method correlated well with the conventional colorimetric method (r=0.968).}}

訂正日: 2006/06/30 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

Peroxyoxalate chemiluminescence (CL) assay of hydrogen peroxide (H₂O₂) or glucose was developed by using 2, 4, 6, 8-tetrathiomorpholinopyrimido[5, 4-d]pyrimidine as a fluorescent component and bis(2, 4, 6-trichlorophenyl)oxalate (TCPO) as an oxalate. Linear relationships between CL intensity and final concentration of H₂O₂ from 10^-8 to 10^-4M were obtained. The detection limit at the ratio of CL intensities for sample and blank (S/B) of 3 was 10nM. The precision for five replicate measurements at 10^-5 and 10^-6M of H₂O₂ were 17.6 and 15.7% of relative standard deviations, respectively. α-D-Glucose was transformed to β-D-glucose with mutarotase and converted to H₂O₂ and D-gluconic acid with glucose oxidase, which was detected by using peroxyoxalate CL reaction. A linear calibration graph was obtained up to 1.5×10^-4M of glucose solution. The method was applied to the assay of glucose in human serum. The recovery was 98.2% (n=4). The method correlated well with the conventional colorimetric method (r=0.968).

責任著者(Corresponding author)

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