抄録
A rapid and sensitive flow-injection method with peroxyoxalate chemiluminescence (CL) detection for the determination of glucose and uric acid by using an immobilized enzyme reactor is described. CL produced in a flow cell by the reaction of hydrogen peroxide with bis(2, 4, 6-trichlorophenyl)oxalate and a fluorescent compound, 2, 4, 6, 8-tetrathiomorpholinopyrimido[5, 4-d]pyrimidine, was monitored. Linear responses between the concentration and the CL intensity for glucose and uric acid were obtained up to 3×10-4M (6nmol per 20μl injection, r=0.999) and 1×10-5M (0.2nmol per 20μl injection, r=1.000), respectively; both limits of detection were the same (5×10-7M, 10pmol per 20μl injection, S/N>3). The relative standard deviations for ten replicate measurements were 1.1% for glucose (2×10-5M, 0.4 nmol/injection) and 2.2% for uric acid (5×10-6M, 0.1nmol/injection). Good correlations between the results of serum glucose or uric acid assay by the proposed methods and by the conventional colorimetric methods (r=0.994for glucose and r=0.986 for uric acid) were obtained.
